Preparation of pH 3.0 Agar Plate, Enumeration of Acid-Tolerant, and Al-Resistant Microorganisms in Acid Soils
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概要
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We attempted to prepare an agar plate containing Al under the acid conditions (pH 3.0) to count the number of colonies of Al-resistant microorganisms and isolate them. As a result, Al-resistant microorganisms were isolated from the tea garden soils and identified. 1) Agra could be solidified when the culture medium solution (pH 3.0), Al_2(SO_4)_3 solution (pH 3.0), and agar solution were mixed after autoclaving separately. 2) The number of Al-resistant microorganisms was largest in the strong acid tea garden soil, followed by the forest soil and smallest in the upland soil. In particular, the number of Al-resistant microorganisms (Al 100 mM in agar medium) accounted for 12.5% of the number of total microorganisms, suggesting that the accumulation of Al-resistant microorganisms increased when the soil pH decreased. 3) Fungi accounted for most of the high Al-resistant microorganisms. 4) About half of the acid-tolerant fungi (grown at pH 3.0) from the tea garden soils consisted of high Al-resistant microorganisms (Al ; 100 mM). 5) Eight strains Emericellopsis sp., Paecilomyces lilacinus, Moritierella ramanniana var. angulispora, Sprothrix inflata, Penicillium glabrum, Metarhizum anisopliae, Chaetospharia inaeqalis, and Aspergillus fumiatus were identified as Al-resistant fungi from the tea garden soils.
- 社団法人日本土壌肥料学会の論文
著者
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KANAZAWA Shinjiro
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Kunito T
Faculty Of Agriculture The University Of Tokyo
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Kunito Takashi
Faculty Of Agriculture The University Of Tokyo
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Kanazawa Shinjiro
Department Of Agricuitural Chemistry Faculty Of Agriculture The University Of Tokyo
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Kanazawa Shinjiro
Department Of Environment Science And Technology Faculty Of Agriculture Kagoshima University
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