Effect of Tris-HCl Buffer on DNA Adsorption by a Variety of Soil Constituents

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概要

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• We investigated the effect of tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer (pH 7.0) as a bulk solution on the adsorption of DNA by gibbsite, goethite, montmorillonite, kaolinite, synthetic and natural allophanes, two humic acids and two andosols. The natural allophane, gibbsite, kaolinite and an andosol adsorbed significantly more DNA in a 0.1 M Tris-HCl buffer than in a 0.1 M NaCl solution (t-test, P<0.005). In contrast, montmorillonite adsorbed significantly less DNA in the Tris-HCl than NaCl solution (P<0.05). Care should be taken when using Tris-HCl in studies on the adsorption of extracellular DNA molecules by soil particles.

• 日本微生物生態学会・日本土壌微生物学会の論文

著者

• SAEKI KAZUTOSHI

  Biotron Institute, Kyushu University

• SAKAI MASAO

  Faculty of Agriculture, Kagoshima University

• Kunito Takashi

  Faculty Of Agriculture The University Of Tokyo

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• FLUORIDE REMOVAL BY A VARIETY OF MINERALS IN AQUEOUS PHASE
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