Hard tissue formation by cultured dental pulp cells and bone marrow cells

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Under certain conditions, undifferentiated mesenchymal cells in bone marrow induce bone-like tissue in a porous apatite scaffold in vitro and in vivo. These cells in the pulp may also play a role in hard tissue formation as bone marrow cells. We evaluated hard tissue formation by dental pulp cells and bone marrow cells with and without a regulator. Hard tissue formation was also evaluated in the presence of a calcium phosphate cement and a new ceramic disk. We cultured pulp cells obtained from the mandibular incisors of rats, and bone marrow cells obtained from rat femurs. We observed active formation of hard tissue by dental pulp cells in vitro. However, formation by bone marrow cells required dexamethasone. An in vivo study using a calcium phosphate cement in mandibular incisors demonstrated no hard tissue formation in the cement. Although hard tissue was formed in the new calcium metaphosphate fiber disk by bone marrow cells in the presence of dexamethasone, there was no tissue formation in the calcium phosphate cement. We concluded that although the calcium metaphosphate fiber disk could be used as a scaffold, calcium phosphate cement could not.
大阪歯科学会の論文