塩素処理した水試料中に含まれる変異原物質を捕捉するための前処理方法の検討

概要

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The pretreatment procedures for measuring the mutagenic activity of water samples were examined. In order to remove the residual chlorine, N_2 gas bubbling and sodium thiosulfate addition were performed. The former was not effective on the mutagenic activity in the Ames test, but the later reduced such activity. The storage methods before and after concentration were compared. The mutagenic activity of the sample, which had no chlorine removed and was kept at 4℃ for 1 month before concentration, was greater than that of chlorine was not stored. There was no difference in the mutagenicity between the sample treated with N_2 gas and kept at-80℃ for 1 month after concentration, and the control sample. The solvents for elution of the adsorvents on a XAD-2 column were also evaluated and discussed. The extract eluted with ethyl acetate showed higher mutagenic activity than the extract eluted with dichloromethane. From these results, we recommend that water after chlorination is first treated with N_2 gas and then applied to an XAD-2 resin column ; the adsorvents are then best removed by eluting with ethyl acetate.
日本環境変異原学会の論文
2000-02-28