ヒト精子を用いた染色体突然変異原性試験の最近の状況

概要

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Recent aspects of chromosomal mutagenicity testing with human spermatozoa were reviewed with emphasis on the development of new research techniques. These include(1)sperm chromosomal mutagenicity test of chemical metabolites by applying a metabolic activator, S9(microsomal fraction of rat liver cells), (2)Detection of mutagen-induced sperm chromosome translocations using a chromosome painting method, (3)micronucleus test in 2-cell embryos after in vitro fertilization of hamster oocytes with mutagen-treated human spermatozoa, (4)detection of aneuploid human spermatozoa using multicolor FISH(fluorescence in situ hybridization)on sperm nuclei, and(5)chromosome analysis of human spermatozoa with aberrant head morphologies after microinjection of the sperm into mouse oocytes. Outline of these methods and new findings thereby obtained were briefly mentioned. The merit and dimerit of these methods and their applicability to genetic risk assessment in humans are also discussed.
日本環境変異原学会の論文
1998-07-21