Blood Cardioplegia Recovers Myocardial Viability after Simple Cold Immersion in Long-term Heart Preservation

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Background & Aims : We studied the efficiency of blood cardioplegic (BCP) solution as a perfusate in a combined method for long-term heart preservation. Methods: Fourteen adult mongrel dogs' donor hearts were stored in a hypothermic (4℃) University of Wisconsin (UW) solution for 12 hours and divided into two groups by the type of coronary perfusates : 15℃ BCP solution (n=7), and 4℃ UW solution (n=7). High-energy phosphate components (phosphocreatine, β-adenosine triphosphate) and intracellular pH were measured by ^31P-nuclear magnetic resonance spectroscopy. Creatine phosphokinase activity and lactic acid value discharged from the coronary sinus during the period of coronary perfusion were also measured. Results : BCP perfusion after simple cold immersion provided significant restoration of phosphocreatine and β-adenosine triphosphate, and lower levels of creatine phosphokinase and lactic acid compared with the perfusion using the UW solution. On the other hand, water content was significantly increased after coronary perfusion using BCP solution. However, there were no significant differences in histological findings between hearts perfused by either perfusate. Conclusion : These results suggest that blood cardioplegic solution is preferable to the UW solution for coronary perfusion following simple cold immersion. (Kitakanto Med J 2003 ; 53 : 243〜249)
北関東医学会の論文
2003-08-01