Internal Standard Compounds for Quantitative Determination of Bile Acids by Gas Chromatography

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概要

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• Gas chromatography is well recognized as a useful tool with several advantages for the analysis of bile acids as well as various compounds. In gas chromatographic analysis, bile acids in an analytical sample are subjected to a number of complicated procedures involving many steps such as extraction, fractionation, solvolysis, hydrolysis, derivatization and injection to the gas chromatograph. These procedures result in the loss of bile acids in the analytical sample. The addition of suitable internal standard compound(s) into the analytical sample prior to the extraction of bile acids is indispensable for an accurate determination of bile acids. There are two methods for the quantitative determination of bile acids in a biological sample by gas chromatography: one is the determination of total bile acid amounts in the sample. The other is the determination of bile acid amounts in each fraction after group separation of bile acids in the biological sample using an ion exchange gel column. The addition of 7β,12α-dihydroxy-5β-cholanoic acid or 7β,12β-dihydroxy-5β-cholanoic acid as an internal standard compound is useful for the former method. On the other hand, the addition of 7β,12β-dihydroxy-5β-cholanoic acid, glyco-7α,12α-dihydroxy-5β-cholanoic acid, tauro-7α,12β-dihydroxy-5β-cholanoic acid and glyco-7β,12α-dihydroxy-5β-cholanoic acid 7-sulfate is a suitable combination as internal standard compounds for the latter method.

• 鳥取大学の論文

著者

• Yamaga Nobuo

  Department Of Biochemistry And Department Of Pathological Biochemistry Tottori University Faculty Of

• Yamaga Nobuo

  Department Of Biochemistry And *first Department Of Internal Medicine Tottori University Faculty Of

関連論文

• Analysis of Bile Acids in Urine Specimens from Healthy Humans: Detrmination of Several Bile Acids with β-Hydroxyl and Carbonyl Groups
• Uncommon Urinary Excretion Pattern of Bile Acids in Healthy Humans: Identification and Determination of 7β-Hydroxylated Bile Acid Series
• An Examination of Alkaline Hydrolyzing Conditions of Conjugated Bile Acids with Carbonyl Groups
• Utility of 19-Hydroxycholesterol as an Internal Standard Compound for the Quantitative Determination of Sterols Using Capillary Gas Chromatograph
• Internal Standard Compounds for Quantitative Determination of Bile Acids by Gas Chromatography
• Analysis of Bile Acids in Urine Specimens from Healthy Humans: Determination of Several Bile Acids with .BETA.-Hydroxyl and Carbonyl Groups.

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