Utility of 19-Hydroxycholesterol as an Internal Standard Compound for the Quantitative Determination of Sterols Using Capillary Gas Chromatograph
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概要
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Gas chromatography is able to detect many kinds of sterols simultaneously. In order to identify and estimate accurately many kinds of sterols in biological samples by gas chromatography, it is indispensable to add an internal standard compound into the analytical samples prior to the analysis. However, there is no report on a suitable internal standard compound for the determination of sterols. In this study, 19-hydroxycholesterol was proved to be a suitable internal standard compound for simultaneous and quantitative determination of several sterols in various analytical samples by capillary gas chromatography, including the quantitative determination of β-cholestanol in the human plasma. Moreover, the excellent condition of gas chromatographic analysis for quantitative determination of several sterols in various analytical samples was found.
- 鳥取大学の論文
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関連論文
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- An Examination of Alkaline Hydrolyzing Conditions of Conjugated Bile Acids with Carbonyl Groups
- Utility of 19-Hydroxycholesterol as an Internal Standard Compound for the Quantitative Determination of Sterols Using Capillary Gas Chromatograph
- Internal Standard Compounds for Quantitative Determination of Bile Acids by Gas Chromatography
- Analysis of Bile Acids in Urine Specimens from Healthy Humans: Determination of Several Bile Acids with .BETA.-Hydroxyl and Carbonyl Groups.