運動刺激による線維芽細胞の人工骨媒体三次元様増殖

概要

論文の詳細を見る
Hydroxyapatite ceramics (HAP) are widely used as implant materials for periodontal bonedefects because of their excellent biocompatibility. We demonstrated that HAP medicated C3H10T1/2 mouse fibroblasts can from 3-dimensional tissue-like structures when mechanically stimulated. Tripsinized 10T1/2 cells were simultaneously co-cultured with 200 HAP grannies on a rotator for 7 days. Thereafter, the cells were moved to a regular incubator. Cell reaction was observed by phase-contrast microscopy. In 2 to 4 weeks, cells showed a pile-up growth phenotype-like 3-dimensional structure surrounding HAP granules. On the other hand, when mechanical stimuli were delayed for 24 hrs after tripsinization cells ceased proliferation caused by density dependent growth soon afler regular incubation, and became a monolayer culture without pile-up growth around the HAP granules. These results indicate that mechanical stimuli to the connection between cell andbone may stimulate cell proliferation and repair damaged tissue.
硬組織再生生物学会の論文
1998-10-04