A Trace Amount of the Human Pro-apoptotic Factor Bax Induces Bacterial Death Accompanied by Damage of DNA
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概要
- 論文の詳細を見る
An amount of human pro-apoptotic Bax as low as 0.01% of total protein was sufficient to cause cell death in Escherichia coli. The bacterial cell death was examined using a viable bacteria-specific fluorescence indicator system and loss of colony formation ability. Coexpression of anti-apoptotic Bcl-x_L showed a modest inhibitory effect on the cell death caused by Bax. The trace amount of Bax elongated E. coli and accumulated monounsaturated fatty acids, suggesting an unusual metabolism of redox in the host. In fact, an increase of KCN-dependent O_2 consumption accompanied the expression of Bax. At the same time, a fluorescent pH indicator showed the apparent accumulation of protons outside the cell, suggesting that the membrane is intact. Bax increased the level of superoxide anion as measured by the expression of superoxide-dependent promoter. Nicked DNA was significantly generated, and the frequency of mutations resistant to rifampicin was increased by 30-fold, depending upon the expression of Bax. It is proposed that trace amounts of Bax increase oxygen consumption, triggering generation of superoxide, which affects DNA, leading to bacterial death.
- 日本医科大学の論文
- 1999-03-25
著者
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Ohta Shigeo
Department of Biochemistry and Cell Biology, Institute of Development and Aging Science, Graduate Sc
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Ohta Shigeo
Department Of Biochemistry And Cell Biology Institute Of Development And Aging Science Graduate Scho
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NISHIMAKI KIYOMI
Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate S
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ASOH Sadamitsu
Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate S
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Nishimaki Kiyomi
Department Of Biochemistry And Cell Biology Institute Of Gerontology Nippon Medical School:(present
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Nishimaki Kiyomi
Department Of Biochemistry And Cell Biology Institute Of Development And Aging Sciences Graduate Sch
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Asoh Sadamitsu
Department Of Biochemistry And Cell Biology Institute Of Development And Aging Sciences Graduate Of
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Asoh Sadamitsu
Department Of Biochemistry And Cell Biology Institute Of Gerontology Nippon Medical School:(present
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Nanbu-Wakao Rika
Department of Biochemistry and Cell Biology, Institute of Gerontology, Nippon Medical School
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Nanbu-wakao Rika
Department Of Biochemistry And Cell Biology Institute Of Gerontology Nippon Medical School:(present
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Ohta Shigeo
Department Of Biochemistry And Cell Biology Institute Of Gerontology Nippon Medical School:(present
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