TISSUE CULTURE OF SOME ANTARCTIC LICHENS PRESERVED IN THE REFRIGERATOR (Eleventh Symposium on Polar Biology)

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Cultured tissues were obtained for the first time from the frozen thalli of three Antarctic lichens, Rhizocarpon flavum DODGE & BAR., Umbilicaria aprina NYL., and U. decussata (VILL.) ZAHLBR. preserved in a refrigerator for nearly five years. The effect of temperature on their growth was determined by means of tissue culture. For comparison, cultured tissues of two Japanese Umbilicaria species, U. muehlenbergii (Acn.) TUCK, and U. pennsylvanica HOFFM., were used. All cultured tissues treated, except those of U. aprina, show better growth at 15℃ than at 5℃. Only U. aprina (mycobiont) grows better at 5℃ than at 15℃ and it seems to be strongly adapted to cold temperature; it grows five times its initial weight at 5℃ after 16 weeks culture, while at 15℃ the same species grows twice its initial weight.
国立極地研究所の論文