<原著>核酸の電解合成

概要

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Electrochemical removal of allylic protecting groups of nucleosides and nucleotides in conjunction with organo-palladium chemistry is described. Choice of the supporting electrolyte, depending on the nature of the products, is crucial in obtaining operational simplicity of the product isolation. In the reaction producing a water-insoluble compound, use of water-soluble Et_4NOTs as the electrolyte is recommended, where the product can be separated very easily from the electrolyte by simple extraction. On the other hand, a hydrophobic electrolyte like (n-C_4H_9)_4NPF_6 should be employed in the reaction providing a water-soluble product. In such cases, the water-insoluble electrolyte and the Pd(0) catalyst can be removed at once by extraction in an organic layer. This method required no tedious, time-consuming chromatographic treatment for isolation of the products, allowing operationally simple preparation of nucleotides.
鈴鹿医療科学大学の論文
1996-03-30