Research of a Bombyx Humoral Lectin Activating Factor by FPLC

概要

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The result of the haemagglutination assay showed that the mature larval haemolymph fraction and the pupal fat body fraction had the ability to activate the inactive lectin-protein (130K-glycoprotein). The fact suggests the possibility that an enzyme activating the inactive lectin-protein presents in the fat body, that the inactive one is activated with the enzyme in the fat body, and that the active one is moved into the haemolymph. Accordingly, we tried gel filtration on a Superdex 200 column for researching neuraminidase in the fat body of Bombyx mori. The result of gel filtration showed that the crude enzyme fraction was obtained from the pupal fat body. Moreover, the result of the colour reaction by means of the thiobarbituric acid assay suggested the possibility that neuraminidase as the enzyme activating the inactive lectin-protein presented in the crude enzyme fraction obtained from the pupal fat body fraction by gel filtration. This research was partly supported by a grant from Tezukayama Gakuen.
帝塚山大学の論文
1998-03-01