Contribution of Glycosidases to Lectin Activity in Haemolymph of Bombyx mori

概要

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The contribution of glycosidases, neuraminidase and galactosidase, to the lectin activity in the haemolymph of Bombyx mori was investigated. The neuraminidase activity was estimated according to determine sialic acid separated from N-acetyl neuramin lactose treated with the haemolymph of Bombyx mori, by means of high performance liquid chromatography (HPLC). Namely, the analysis of sialic acid was performed by using the ISA-07/S2504 column this time, while the analysis of sialic acid was performed by using the SCR 101H column until now. On the other hand, the galactosidase activity was estimated according to determine the content of D-galactose in the active lectin and in the inactive one by HPLC, comparatively. The results suggested that neuraminidase and galactosidase were activated with the ecdyson in the mature larval haemolymph of Bombyx mori, therefore that the appearance and the disappearance of the lectin activity were controled with these enzymes. This research was partly supported by a grant from Tezukayama Gakuen.
帝塚山大学の論文
1995-02-01