口蓋形成術術後の上顎歯槽部,口蓋粘膜の知覚に関する研究第2報幼若ラットの口蓋粘膜切除後の神経再生に関する免疫組織化学的研究

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The purpose of this study was to examine nerve growth associated with the healing process of two types of palatal wounds. Nerve growth in palatal wound following palatoplasty was studied for up to 12 weeks postoperatively using immunohistochemical techniques. For the purpose of immunohistochemical analysis two groups of rats were created according to the degree and area of surgical excision. Group 1 involved a partial-thickness excision of mucosa (supraperiosteal) with preservation of the mucoperiosteum. Group 2 involved a full-thickness excision of mucosa including the mucoperiosteum. Palatal area (2.5×5mm) was removed along the left-maxillary first and second molars for both groups of the rats at 3 weeks of age. After 1, 3, 5, 7, 14, 21, 28, 58 and 86 days of healing, decalcified material including the alveolar bone was sectioned and stained by means of H.E and immunohistochemistry for detection of the neurofilament protein (NFP). In group 1, nerve growth was first detected by NFP immunoreactivity seen at between 3 to 5days. At 21 days, regenerated nerve fibers were clearly observed reaching beneath the epithelium. An increase in the number of regenerated nerve fibers was seen beneath the epithelium until 21 days, but after that, the number decreased gradually until day 86. In group 2, the denuded bone surface was completely covered by regenerated squamous epithelium at 14 days. Regenerated nerve fibers were, however, observed only in the regenerated loose connective tissue adjacent to the excisional portion. No regenerated nerve fibers in the epithelium were found in the dense collagenous part of the connective tissue. The number of regenerated nerve fibers did not increase from day 1 postoperatively until day 86. In conclusion, it can be stated that surgical preservation of periosteum and connective tissue in the palate resulted in a rich nerve growth in the regenerated mucosal tissue. When the mucoperiosteum was excised, however, the nerve growth in the developine submucosal scar tissue was poor.
札幌医科大学の論文
2000-12-01

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