Staphylococcus aureusの糖の燐酸化的膜透過機構 : トルエン処理菌浮遊液における ONPG分解反応

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概要

• 論文の詳細を見る

• Intact cells of Staphylococcus aureus, strain AF 121,which had been grown in galactosecontaining broth, hydrolyzed o-nitrophenyl-β-D-galactoside (ONPG), a lactose analog, and released o-nitrophenol (ONP) into the medium. The activity was totally lost by treating the cells with toluene, but it was restored when phosphoenolpyruvate (PEP) was supplemented to the medium. Therefore, this method can be conveniently used for demonstrating the requirement of PEP for the ONPG-hydrolysis reaction in this organism. The activities obtained varied with conditions of the treatment. The highest activity was observed when the cells were treated with 6μl of toluene per ml for 30 min at 37℃.

• 高知学園短期大学の論文
• 1977-04-30

著者

• 藤沢 麻子

  高知学園短期大学術生技術科微生物学研究室

関連論文

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• ブドウ球菌におけるラクトースリン酸化酵素系の分布
• Staphylococcus aureusの糖の燐酸化的膜透過機構 : トルエン処理菌浮遊液における ONPG分解反応
• ヒトの口腔粘膜より分離したブドウ球菌の糖分解能
• Vibrio parahaemolyticusのphosphoenolpyruvate-dependentphosphotransferase system (PEP・PTS)

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