<原著>上皮成長因子EGFによる退縮型癌細胞ER-1の浸潤,転移能の促進に関する研究

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Tumor cells always interact with host reactive cells in the process of carcinogenesis and tumor growth. Likewise, malignant phenotypes o tumor cells are affected by cytokines and growth factors released from surrounding host reactive cells This study examined the effects of epidermal growth factor (EGF) on tumorigenicity, invasive ability and the metastatic potential of regressor ER-1 cells isolated from a spontaneously developed SHR mammary adenocarcinoma cell line. When ER-1 cells were pretreated with EGF(10ng/ml) for 24hours(24h EGF ER-1), the invasive ability was enhanced in in vitro invasion assay using rat lung endothelial cells (RLE). The invasion activity enhanced by EGF was reversible fashion and decreased to the level of non-treated ER-1 cells on day 4. However, ER-1 cells cultured in the presence of EGF for one month (1M EGF ER-1) kept a high invasive ability for two months. In in vivo pulmonary metastasis and intraperitoneal inoculation experiments, ER-1 cells treated with EGF were found many tumor nodules in the lung and mesenterium as compared with non-treated ER-1 cells 24h EGF ER-1 cells showed a temporary enhancement of invasive abilities and metastatic potentials, but 1M EGF ER-1 cells showed a fixed malignant phenotype for two months In EGF binding activity assay and analysis of EGF receptor number, there were no differences between ER-1 cells and 1M EGF ER-1 cells. No change was observed in the expression of EGF receptor mRNA between ER-1 cells and 1M EGF ER-1 cells by RT-PCR. These results strongly suggest that long term EGF treatment may convert regressor ER-1 cells to progressor cells and that this may cause genomic alteration to ER-1 cells. The study indicate that EGF may play an important role in the acquisition of malignant phenotypes of tumor cells.
北海道医療大学の論文
1994-12-31

<原著>上皮成長因子EGFによる退縮型癌細胞ER-1の浸潤,転移能の促進に関する研究

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