原子吸光分析法によるカルシウムの定量について
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概要
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In recent times Atomic absorption spectrometry has been widely used as an analytical method for the heavy metals such as platinum and also has been applied in the analysis of other metals. Hereafter, an even broader application of this method is expected. Although it has not yet been re-cognized as an official method of analysis for the determination of calcium, the atomic absorption method has been used recently more and more because of its ability to conveniently analyze several samples in a short time. In order to shed light on the problems rising from the standpoint of precision and, more importantly, to obtain a deeper knowledge of the method of atomic absorption spectrometry, this experiment was carried out. Methods and Materials The femur, tibia, and fibula of rats of the Wistar strain were used. For purposes of comparative analysis the following were used : Two methods of preparing a sample solution; two methods of analyzing the results, namely, the calibration curve method and the standard addition methods ; and two different types of flames, namely, a nitrous oxide-acetylene flame and an air-acetylen flame. The fat of the femur, tibia, and fibula was removed by means of the Soxhlet extraction with ethyl ether for 20 hours. The de-fatted bone was incinerated at 600℃ until white ash was obtain-ed. Some of the bone ash was then dissolved in a minimal amount of 1 : 4 HCl and increased to a suitable volume by means of de-ionized water. Also, the remaining bone ash was dissolved in a l : 1 solution of HCl and then evapolated to dryness. After filtration it was diluted by means of de-ionized water. The results of the two solutions were then compared. Results The results in determining animal bone calcium were the same for both sample solutions, that is, for the bone ash obtained by dry incineration and dissolved in a l : 4 HCl solution, and that which was diluted by deioni, zed water after previous manipulation. As for the calibration curve method and the standard deviation method, the results were the same, provided the dry incineration was sufficient. Since a large number of samples can be analyzed in a short time using the calibration curve method, it is preferred for its convenience. In comparing the nitrous oxide-acetylene flame with the air-acetylene flame, it was discovered that in the case of the former, a straight line was more easily obtained on the calibration curve. In the case of the air-acetylene flame, if the solution was diluted sufficiently, a straight line was obtain-ed. However, with concentrations above 3ppm, a straight line was not obtained, thereby severely limiting the sensitivity of the analysis.
- 聖霊女子短期大学の論文
- 1975-12-20