<予防および治療>エネルギー枯渇心に対する心筋保護, 特にterminal warm blood cardioplgiaについての検討

概要

論文の詳細を見る
The effect of temperature of terminal warm blood cardioplegia (TWBCP) on the energy metabolism was evaluated with ^<31>P-NMR and HPLC using isolated rabbit hearts perfused by Krebs-Henseleit bicarbonate buffer with Langendorff mode. Fifty percent depletion of myocardial ATP (energy-depleted heart) was produced by 30 minutes global ischemia at 37℃ in 17 isolated rabbit heart. Subsequently, cardioplegic arrest was made with cold blood cardioplegia at 10℃ administered every 20 minutes. Just before reperfusion, TWBCP with a temperature at 10℃ (TWBCP-10 : n=7), 25℃ (TWBCP-25 : n=5) and 37℃ (TWBCP-37 : n=5) were administered. However, just after the rererfusion, TWBCP-37 showed significantly prompt recovery of CP to other groups. After 30 minutes of reperfusion, no significant difference in recovery of CP existed between the three groups. ATP was not recovered after reperfusion. Coronary sinus effluent was analyzed with HPLC, and 1.90±0.69μmol inosine was washed out from ischemic myocardial cell during cardioplegic arrest. We conclude that use of TWBCP at 37℃ promotes the myocardial CP recovery in the energy-depleted heart, but if myocardial nucleotides have been washed out before TWBCP, ATP content can not be recovered at all.
近畿大学の論文
1991-12-30