<原著>培養角膜上皮細胞の伸展における細胞内骨格の役割について

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概要

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Corneal epithelial resurfacing is the first step of corneal wound healing. In the normal cornea, fibronectin is not detected in the epithelial basement membrane. When corneal epithelial cells are removed mechanically, fibronectin appears firstly on the denuded corneal surface. Remaining cells adjacent to a wound spread and migrate over the fibronectin matrix. Generally, cytoskeleton is important in cell movement, internal organization and cell shape. To understand the role of cytoskeleton in the corneal epithelial resurfacing, rabbit corneal epithelial cells were cultured on fibronectin matrix and the localization of actin filaments and microtubules in the spread cells were investigated by fluorescent microscopy. To investigate the roles of actin filaments and microtubules quantitatively, cytochalasin B and colchicine were added sometimes to the culture medium, which are known to inhibit the assembly of actin filaments and microtubules respectively. Then the changes of the area of spread cells were investigated. The cultured rabbit corneal epithelial cells spread well on fibronectin matrix. Actin filament bundles were observed throughout the cytoplasm of spread cells, whereas microtubules were observed only at the perinuclear region of the cytoplasm. Generally, the area of cultured corneal epithelial cells increased in proportion to the concentration of fibronectin. The addition of cytochalasin B decreased the area of spread cells significantly. The addition of colchicine decreased the area of spread cells only slightly. It seems to be demonstrated that the formation of actin filaments is important for the corneal epithelial wound healing.
1989-03-25

著者

福田昌彦
近畿大学医学部眼科学教室

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