IL-12 AND IL-10 RELEASED FROM PERIPHERAL BLOOD MONONUCLEAR CELLS IN SARCOIDOSIS IS ASSOCIATED WITH DIFFERENTIAL MODULATION or B7-1 (CD80) AND B7-2 (CD86) EXPRESSION ON MONOCYTES

概要

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Sarcoidosis is a systemic disorder of unknown etiology, in which noncaseating granulomas simultaneously occur in several organs and cell mediated type 1 immune responses are considered to play critical roles in the pathogenesis. The aim of this study was to ascertain whether the initial immune response developed by peripheral blood mononuclear cells (PBMCs) from patients with active sarcoidosis might induce the cell mediated type l immune response observed at disease sites. We evaluated IL-12 and IL-10 production in PBMCs on priming with LPS and/or INF-γ using enzyme immunosorbent assay (ELISA) and the expression of the co-stimulatory molecules, CD80 and CD86,on the surfaces of monocytes by flow cytometric analysis (FCM) in 11 patients with active sarcoidosis. PBMCs from patients released the increased levels of IL-12,in contrast, the decresed levels of IL-10 as compared with those from healthy subjects. Analysis by FCM of CD14^+ monocytes from patients revealed the overexpression of CD86 compared with healthy subjects, while no significant difference of CD80 expression was observed. We found that the expression of CD86 was inversely correlated with IL-10 production. The results suggest that sarcoid CD14^+ monocytes may induce cell mediated immune responses under conditions of increased IFN-γ concentration, such as at disease sites.
名古屋市立大学の論文
2001-09-01