IMMUNOELECTRON MICROSCOPIC STUDY FOR THE INFLUENCE OF LH-RH BY INTERCONNECTED FOLLICULO-STELLATE CELLS
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概要
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Intercellular gap junctions between folliculo-stellate cells of anterior pituitary glands indicate the existence of cell-to-cell communication within the organ. In the present study, we propose a possible role for regulation of hormone secretion in the anterior pituitary gland under castrated conditions. Twenty Wistar-Imamichi strain male rats were separated into 2 groups. Whereas the first group was for not-castrated group, the second group was castrated on day 10,and each group was sub-divided into 2 subgroups as controls and LH-RH treated groups. The animals were killed on day 40,whose calvaria were opened and brains were removed. In case of LH-RH treatment groups, the animals were treated with LH-RH for 3 min, while in control groups this LH-RH treatment was omitted. Then, the pituitary glands were processed for immunohistochemical light microscopy and immunoelectron microscopy. The reaction end-products were observed around the vessels especially in the pericapillary area outside of the cell membrane only in castrated LH-RH treated rats at the light microscopic level. A few electron dense particles sized approximately 100-150 nm in diameter appeared in the peri-capillary space by using electron microscopy. The immunoelectron microscopic technique by using protein A gold showed that these electron dense particles contained LHβ immunoreactive substance. I suppose that the stimulatory "message" of LH-RH can be transmitted via the folliculo-stellate cells to the granulated cells in the anterior lobe by the gap junctionally connected network to affect LH release from gonadotrophs in castrated rats.
- 名古屋市立大学の論文
- 2000-11-01
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関連論文
- FUNCTIONAL CHANGES OF RAT SYNOVIAL LINING CELLS WITH POSTNATAL DEVELOPMENT
- INTER-CELLULAR COMMUNICATIONS IN THE RAT DORSAL ROOT GANGLIA
- IMMUNOELECTRON MICROSCOPIC STUDY FOR THE INFLUENCE OF LH-RH BY INTERCONNECTED FOLLICULO-STELLATE CELLS