<Originals>An experimental study of wound healing at the site of microvascular anastomosis in special reference to endothelial recovery

概要

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Re-endothelialization of the rat femoral artery following end-to-end microvascular anastomosis was investigated by using a scanning electron microscope (SEM) and a light microscope (LM). The microvascular wound healing was schematically quantified from the SEM images using a computerized image processing system. The results revealed that the re-endothelialization process was divided into eight successive processes ; starting with the initial repair of four stages of the intimal lesion at the base of the thread and continuing on the interdigitated four phases of the embedding of the suture. Based on our quantitative computer analysis, it was revealed that the density of the fibrin network gradually increased until the third postoperative day. This process was succeeded by a sigmoid increase of the endothelial growth over the intimal rupture at the base of the suture and continued to extend over the exposed thread surface at the lumen of the vessel. This study details that the exaggerated endothelial impairment and the protuberant thread in the luminal surface delays the re-endothelialization. The significance of the findings which were obtained at small intervals to understand the process of re-endothelialization lies in relation to the identification of the time of thrombus formation, the knowledge of which allows us to better treat the microsurgically injured endothelium and to prevent microvascular occlusion.
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