Characterization of Two Isozymes of Coniferyl Alcohol Dehydrogenase from Streptomyces sp. NL15-2K
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概要
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We purified two isozymes of coniferyl alcohol dehydrogenase (CADH I and II) to homogeneity from cell-free extracts of Streptomyces sp. NL15-2K. The apparent molecular masses of CADH I and II were determined to be 143 kDa and 151 kDa respectively by gel filtration, whereas their subunit molecular masses were determined to be 35,782.2 Da and 37,597.7 Da respectively by matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Thus, it is probable that both isozymes are tetramers. The optimum pH and temperature for coniferyl alcohol dehydrogenase activity were pH 9.5 and 45 °C for CADH I and pH 8.5 and 40 °C for CADH II. CADH I oxidized various aromatic alcohols and allyl alcohol, and was most efficient on cinnamyl alcohol, whereas CADH II exhibited high substrate specificity for coniferyl alcohol, and showed no activity as to the other alcohols, except for cinnamyl alcohol and 3-(4-hydroxy-3-methoxyphenyl)-1-propanol. In the presence of NADH, CADH I and II reduced cinnamaldehyde and coniferyl aldehyde respectively to the corresponding alcohols.
- 社団法人 日本農芸化学会の論文
- 2011-09-23
著者
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Davies Julian
Department Of Microbiology And Immunology Life Sciences Institute University Of British Columbia
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Davies Julian
Department Of Microbiology And Immunology Life Sciences Centre University Of British Columbia
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NISHIMURA MOTOHIRO
Department of Cardiovascular and Thoracic Surgery, Kyoto Prefectural University of Medicine
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Kohno Kunie
Department Of Dermatology Shimane University Faculty Of Medicine
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Nishimura Yoshio
Department Of Organic Chemistry Graduate School Of Pharmaceutical Sciences Tohoku University
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Inagaki Masanori
Department Of Pharmaceutical Chemistry Faculty Of Pharmacy Yasuda Women's University
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Nishimura Yoshio
Department Of Pharmaceutical Chemistry Faculty Of Pharmacy Yasuda Women's University
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Kohno Kunie
Department Of Medical Pharmacy Faculty Of Pharmacy Yasuda Women's University
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Nishimura Motohiro
Department Of Pharmaceutical Chemistry Faculty Of Pharmacy Yasuda Women's University
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Inagaki Masanori
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
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DAVIES Julian
Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia
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Nishimura Yoshio
Department of Applied Chemistry, Faculty of Engineering, Keio University
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