Preserved and Unpreserved 12 Anti-allergic Ophthalmic Solutions and Ocular Surface Toxicity : In Vitro Assessment in Four Cultured Corneal and Conjunctival Epithelial Cell Lines
スポンサーリンク
概要
- 論文の詳細を見る
In the present study, we evaluated the cytotoxicity of anti-allergic ophthalmic solutions in cultured corneal and conjunctival cells, namely SIRC (rabbit corneal epithelium), BCE C/D-1b (bovine corneal epithelial cells), RC-1 (rabbit corneal epithelium), and Chang (human conjunctival cells). The viability of cell cultures was determined following the exposure of cells to 12 commercially available anti-allergic ophthalmic solutions for varying exposure times and at various dilutions using the MTT and neutral red assays. The cell viability score (CVS) was used to compare the toxicity of different drugs. Based on CVS data, the order of cell viability after exposure to the drugs was Zepelin ≥ Tramelas PF ≥ Cumorol PF ≥ Ketotifen PF ≥ Eyevinal = Fumarton ≥ Cumorol > Intal ≥ Rizaben ≥ Tramelas ≥ Patanol Livostin. In conclusion, cell viability was mostly affected by the concentration of benzalkonium chloride rather than the active component and/or the anti-allergic action of the drug. The CVS was useful in comparing the toxicity of different drugs.
- 日本防菌防黴学会の論文
- 2010-12-10
著者
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Ayaki Masahiko
Department of Ophthalmology, Saitama National Hospital
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Koide Ryohei
Department Of Ophthalmology School Of Medicine Showa University
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Iwasawa Atsuo
Tissue Culture Laboratory Showa University Fujigaoka Hospital
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Yaguchi Shigeo
Department Of Ophthalmology Fujigaoka Hospital Showa University
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IWASAWA ATSUO
Department of Clinical Pathology, Fujigaoka Hospital, Showa University School of Medicine
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Koide Ryohei
Dep. Of Ophthalmology Showa Univ. School Of Medicine
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Ayaki Masahiko
Department Of Ophthalmology Saitama National Hospital
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Iwasawa Atsuo
Department Of Bioengineering Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Tech
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YAGUCHI SHIGEO
Department of Ophthalmology, Fujigaoka Rehabilitation Hospital, Showa University School of Medicine
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