Isolation of anthocyanin-related MYB gene, GbMYB2, from Gynura bicolor leaves

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概要

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• The leaves of field-cultivated Gynura bicolor DC. are reddish purple on the abaxial side and green on the adaxial side. The leaves of cultured G. bicolor plantlets, however, appear almost completely green on both sides. Cultured plantlet leaves treated with sucrose accumulated anthocyanins on the abaxial side. In this study, to investigate the anthocyanin-accumulation mechanism in G. bicolor leaves, we isolated the MYB transcription regulatory gene from the leaves using a degenerate PCR method. The isolated gene, GbMYB2, was approximately 10-fold up-regulated in sucrose-treated leaves. In addition, co-expression of GbMYB2 and bHLH-type transcription factor, GbMYC1, activated GbDFR and GbANS promoters in tobacco leaf protoplasts. These results suggest that GbMYB2 might regulate anthocyanin biosynthesis genes in G. bicolor leaves.

• 日本植物細胞分子生物学会の論文
• 2010-12-25

著者

• SHIMIZU Yasuhiro

  Graduate School of Life Sciences, Toyo University

• SHIMOMURA Koichiro

  Graduate School of Life Sciences, Toyo University

• Shimomura Koichiro

  Graduate School Of Life Sciences Toyo University

• MAEDA Kazuhiro

  San-Ei Gen F.F.I., Inc.

• KATO Mika

  Graduate School of Life Sciences, Toyo University

• Shimizu Yasuhiro

  Graduate School Of Life Sciences Toyo University

• Maeda Kazuhiro

  San-ei Gen F.f.i. Inc.

• Kato Mika

  Graduate School Of Life Sciences Toyo University

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• Identification of Novel Poly-Acylated Anthocyanins from Gynura bicolor Leaves and Their Antioxidative Activity
• Isolation of anthocyanin-related MYB gene, GbMYB2, from Gynura bicolor leaves

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