Cell Cycle-Dependent Priming Action of Granulocyte Colony-Stimulating Factor (G-CSF) Enhances in vitro Apoptosis Induction by Cytarabine and Etoposide in Leukemia Cell Lines
スポンサーリンク
概要
- 論文の詳細を見る
We investigated the priming effect and mechanism of granulocyte colony-stimulating factor (G-CSF) in chemotherapy with low-dose Ara-C and VP-16 for acute myeloid leukemia. We analyzed cell proliferation, apoptosis, and cell cycle in vitro using leukemia cell lines 32Dcl3, U937, HL-60, and Ba/F3. Cell proliferation assays were performed using the Trypan Blue dye exclusion method. For detection of apoptosis, the Annexin V-binding capacity of treated cells was examined by flow cytometry. To evaluate the cell cycle, we used an FITC BrdU Flow kit and conducted analysis by flow cytometry. The combination of Ara-C and VP-16 significantly enhanced the observed effects compared with those of Ara-C or VP-16 alone. Concurrent administration of G-CSF further reduced the cell number and viability of 32Dcl3 and U937 cells, but not of HL-60 and Ba/F3 cells. Apoptotic cells were significantly increased in number by the addition of G-CSF to 32Dcl3 and U937 cells, while G-CSF had no significant effect on HL-60 and Ba/F3 cell lines. The addition of G-CSF significantly decreased the percentage of G0/G1-phase cells and significantly increased that of S-phase cells among 32Dcl3 and U937 cells. No significant effect was observed for HL-60 and Ba/F3 cells. An enhancement was confirmed for the combination of Ara-C, VP-16, and G-CSF for 32Dcl3 and U937 cells but not for HL-60 and Ba/F3 cells. It was thought that this difference was a result of different responses to G-CSF. G-CSF potentiates Ara-C- and VP-16-induced cytotoxicities through apoptosis induction by mobilizing resting G0-G1-phase cells into S phase.
- 日本リンパ網内系学会の論文
- 2010-11-01
著者
-
Saio Masanao
高山赤十字病院 循環器内科
-
MORIWAKI H.
Gifu University School of Medicine
-
MORIWAKI Hisataka
First Department of Internal Medicine, Gifu University School of Medicine
-
Shimazaki M
Department Of Internal Medicine Gifu University School Of Medicine
-
Shimazaki Makoto
岐阜大学 1内
-
Moriwaki H
First Department Of Internal Medicine Gifu University Graduate School Of Medicine
-
Moriwaki Hisataka
岐阜大学 医学部内科学
-
Moriwaki Hisataka
がん・感染症センター都立駒込病院 肝臓内科
-
Moriwaki Hisataka
Dep. Of Gastroenterology Gifu Univ. School Of Medicine
-
Moriwaki Hisataka
東京大学医科学研究所
-
Shiraki Makoto
岐阜大学 医学部内科学
-
Suzui Masumi
Internal Medicine Gifu University School Of Medecine
-
Suzui Masumi
琉球大学 医学部腫瘍病理学
-
Suzui Masumi
琉球大学 医学部病理学
-
Hara T
Department Of Pediatrics Graduate School Of Medical Sciences Kyushu University
-
Hara Toshiro
九州大学 医研究院成長発達医学
-
SHIMIZU Masahito
First Department of Pathology, Gifu University School of Medicine
-
TSURUMI HISASHI
First Department of Internal Medicine, Gifu University Hospital
-
KANEMURA NOBUHIRO
First Department of Internal Medicine, Gifu University School of Medicine
-
Hara Toshiro
Department Of Child Neurology Institute Of Neurological Sciences Faculty Of Medicine
-
Kitagawa Jun-ichi
Department Of Internal Medicine Kisogawa Municipal Hospital
-
Hara Takeshi
First Department Of Internal Medicine Gifu University Graduate School Of Medicine
-
Mohri Hiromi
1st Department Of Internal Medicine Gifu University School Of Medicine
-
Takao Hiroshi
Second Department Of Surgery Gifu University School Of Medicine
-
Moriwaki Hisataka
First Department Of Internal Medicine Gifu University Graduate School Of Medicine
-
Kanemura Nobuhiro
First Department Of Internal Medicine Gifu University Graduate School Of Medicine
-
Shimizu Masahito
Department Of Medicine Gifu University Graduate School Of Medicine
-
KASAHARA Senji
First Department of Internal Medicine, Gifu University Graduate School of Medicine
-
Tsurumi Hisashi
First Department Of Internal Medicine Gifu University Graduate School Of Medicine
-
Kasahara Senji
First Department Of Internal Medicine Gifu University Graduate School Of Medicine
-
Takada Hidetoshi
Department Of Pediatrics Graduate School Of Medical Sciences Kyushu University
-
Moriwaki H
Gifu Univ. School Of Medicine Gifu Jpn
-
Moriwaki H.
First Department Of Internal Medicine Gifu University School Of Medicine
-
Saio Masanao
Department Of Medicine Gifu University Graduate School Of Medicine
-
Tsurumi Hisashi
Department Of Medicine Gifu University School Of Medicine
-
Shimizu Masahito
First Department Of Internal Medicine Gifu University Graduate School Of Medicine
-
KITAGAWA Junichi
First Department of Internal Medicine, Gifu University Graduate School of Medicine
-
Moriwaki H.
Department Of Internal Medicine Gifu University
-
Shiraki Makoto
First Department Of Internal Medicine Gifu University School Of Medicine
-
Shimizu Masaru
Department Of Medicine Gifu University Graduate School Of Medicine
-
Moriwaki Hisataka
Department Of Medicine Gifu University Graduate School Of Medicine
関連論文
- A novel R275X mutation of the SLC25A15 gene in a Japanese patient with the HHH syndrome
- Role of CD44 in CTL-induced acute liver injury in hepatitis B virus transgenic mice
- Histological and immunohistochemical observations of mucin-depleted foci (MDF) stained with Alcian blue, in rat colon carcinogenesis induced with 1, 2-dimethylhydrazine dihydrochloride
- Double filtration plasmapheresis and interferon combination therapy for chronic hepatitis C patients with genotype 1 and high viral load
- Timing of interferon therapy and sources of infection in patients with acute hepatitis C
- [症例報告]Pulmonary Hypoplasia and Chondrocyte p21^ Expression in Patients with Lethal Short-limbed Dwarfism
- Varicella-Zoster Virus Encephalitis in a Patient Undergoing Unrelated Cord Blood Transplantation for Myelodysplastic Syndrome-Overt Leukemia
- Early-Onset Thyrotoxicosis after Unrelated Cord Blood Transplantation for Acute Myelogenous Leukemia
- Unrelated Cord Blood Transplantation with a Reduced-Intensity Conditioning Regimen following Autologous Transplantation for Multiple Myeloma
- Dietary Prevention of Azoxymethane-Induced Colon Carcinogenesis with Rice-Germ in F344 Rats.
- β-Catenin (Ctnnb1) Gene Mutations in Diethylnitrosamine (DEN)-induced Liver Tumors in Male F344 Rats
- Inhibitory effect of dietary monoglucosylceramide 1-O-β-glucosyl-N-2'-hydroxyarachidoyl-4,8-sphingadienine on two different categories of colon preneoplastic lesions induced by 1,2-dimethylhydrazine in F344 rats
- Genetic alterations in a patient with Turcot's syndrome
- CARCINOGENICITY EXAMINATION OF 1-NITROPYRENE OXIDES AND RELATED CHEMICALS : LACK OF THEIR TUMORIGENIC EFFECTS IN A NEWBORN MICE ASSAY
- Association of IL12RB1 Polymorphisms with Susceptibility to and Severity of Tuberculosis in Japanese : a Gene-based Association Analysis of 21 Candidate Genes
- [総説]Effects of acyclic retinoid on cell cycle progression and signal transduction
- Suppression of Occurrence and Advancement of β-Catenin-accumulated Crypts, Possible Premalignant Lesions of Colon Cancer, by Selective Cyclooxygenase-2 Inhibitor, Celecoxib
- Pitavastatin inhibits azoxymethane-induced colonic preneoplastic lesions in C57BL/KsJ-db/db obese mice
- Long-Term Outcome of Treatment With Protocols AL841, AL851, and ALHR88 in Children With Acute Lymphoblastic Leukemia: Results Obtained by the Kyushu-Yamaguchi Children's Cancer Study Group
- Modifying Effects of Ferulic Acid on Azoxymethane-Induced Colon Carcinogenesis in F344 Rats.
- 出生体重と3歳児体容量指数(body mass index)の関連
- SUPPRESSIVE EFFECTS OF CHLOROGENIC ACID ON N-METHYL-N-NITROSOUREA-INDUCED GLANDULAR STOMACH CARCINOGENESIS IN MALE F344 RATS
- Inhibitory Effects of Nabumetone, a Cyclooxygenase-2 Inhibitor, and Esculetin, a Lipoxygenase Inhibitor, on N-Methyl-N-nitrosourea-induced Mammary Carcinogenesis in Rats
- Esophageal metastasis from prostate cancer: Diagnostic use of reverse transcriptase-polymerase chain reaction for prostate-specific antigen
- Endoscopic ultrasound-guided fine-needle aspiration in patients with lymphadenopathy suspected of recurrent malignancy after curative treatment
- Intrahepatic cholangiocarcinoma arising 10 years after the excision of congenital extrahepatic biliary dilation
- Dietary supplementation with branched-chain amino acids suppresses diethylnitrosamine-induced liver tumorigenesis in obese and diabetic C57BL/KsJ-db/db mice
- Therapeutic effect of intraarterial prednisolone injection in severe intestinal Behcet's disease
- Staphylococcal scalded-skin syndrome in an adult due to methicillin-resistant Staphylococcus aureus
- Overweight and obesity increase the risk for liver cancer in patients with liver cirrhosis and long-term oral supplementation with branched-chain amino acid granules inhibits liver carcinogenesis in heavier patients with liver cirrhosis
- Successful Unrelated Cord Blood Transplantation for Epstein-Barr Virus-Associated Lymphoproliferative Disease with Hemophagocytic Syndrome
- Dominant β-Thalassemia with Hemoglobin Hradec Kralove : Enhanced Hemolysis in the Spleen
- Quantitative Monitoring of Circulating Epstein-Barr Virus DNA for Predicting the Development of Posttransplantation Lymphoproliferative Disease
- Neuron-Specific Enolase in Hemophagocytic Lymphohistiocytosis: A Potential Indicator for Macrophage Activation?
- Phosphorylated retinoid X receptor α loses its heterodimeric activity with retinoic acid receptor β
- Synergistic growth inhibition by acyclic retinoid and vitamin K_2 in human hepatocellular carcinoma cells
- Chemopreventive Effect of N-(2-Cyclohexyloxy-4-nitrophenyl)methane Sulfonamide (NS-398), a Selective Cyclooxygenase-2 Inhibitor, in Rat Colon Carcinogenesis Induced by Azoxymethane
- Mutations of syntaxin 11 and SNAP23 genes as causes of familial hemophagocytic lymphohistiocytosis were not found in Japanese people
- Role of Vα14^+ NKT cells in the development of Hepatitis B virus-specific CTL : activation of Vα14^+ NKT cells promotes the breakage of CTL tolerance
- Immunopathogenesis of hepatic fibrosis in chronic liver injur induced by repeatedly administered concanavalin A
- Reduced intestinal apo A-IV mRNA levels in patients with liver cirrhosis
- Usefulness of MR imaging in the postsurgical monitoring of gallbladder cancer in a patient with bile duct cancer that developed 7 years after resection of mucinous adenocarcinoma of the gallbladder
- Low-Dose Granulocyte Colony-Stimulating Factor Overcomes Neutropenia in the Treatment of Non-Hodgkin's Lymphoma with Higher Cost-Effectiveness
- ENDOSCOPIC NASOBILIARY DRAINAGE : CURRENT INDICATIONS AND EVALUATION OF THE PRODUCTS
- Improvement of fuel metabolism by nocturnal energy supplementation in patients with liver cirrhosis
- Nutritional pharmacotherapy of chronic liver disease : from support of liver failure to prevention of liver cancer
- Both insulin sensitivity and glucose sensitivity are impaired in patients with non-diabetic liver cirrhosis
- Preferential Use of Branched-Chain Amino Acids as an Energy Substrate in Patients with Liver Cirrhosis
- IL-12 induces specific cytotoxicity against regenerating hepatocytes in vivo
- Clearance rate of plasma branched-chain amino acids correlates significantly with blood ammonia level in patients with liver cirrhosis
- Plasma Cell Leukemia with Myelofibrosis
- A Variant Form of Acute Promyelocytic Leukemia with Marked Myelofibrosis
- Attenuation of cardiotoxicity of daunomycin using a complex with heparin
- IgG Type Multiple Myeloma and Concurrent IgA Type Monoclonal Gammopathy of Undetermined Significance Complicated by Necrotizing Skin Ulcers due to Type I Cryoglobulinemia
- Late-Onset Neutropenia in Patients Treated with Rituximab for Non-Hodgkin's Lymphoma
- Graft Failure Due to Hemophagocytic Syndrome After Autologous Peripheral Blood Stem Cell Transplantation
- Long-term outcome of branched-chain amino acid treatment in patients with liver cirrhosis
- Chemoprevention of liver carcinogenesis with retinoids : Basic and clinical aspects
- Oral branched-chain amino acid supplementation improves the oxidized/reduced albumin ratio in patients with liver cirrhosis
- Relationship between Serum Albumin Level and Aging in Community-Dwelling Self-Supported Elderly Population
- ENDOSCOPIC TREATMENT FOR BENIGN BILIARY STRICTURES : CAN PLACEMENT OF A COVERED METALLIC STENT BE AN OPTION IN REFRACTORY CASES ?
- ENDOSCOPIC ULTRASOUND-GUIDED DRAINAGE OF PANCREATIC PSEUDOCYSTS USING A LARGE-CHANNEL ECHOENDOSCOPE AND A CONVENTIONAL POLYPECTOMY SNARE
- Indications for endoscopic papillary balloon dilation for common bile duct stones for the prevention of pancreatitis and the preservation of papillary function after the procedure
- DIAGNOSTIC VALUE OF TRANSPAPILLARY BIOPSY USING DOUBLE LUMEN INTRODUCER FOR DETERMINATION OF MUCOSAL EXTENT IN EXTRAHEPATIC BILE DUCT CANCER
- Therapeutic Effect of Balloon-occluded Retrograde Transvenous Obliteration on Portal-systemic Encephalopathy in Patients with Liver Cirrhosis
- Inflammatory pseudotumor of the liver with primary sclerosing cholangitis
- EUS-GUIDED PANCREATIC PSEUDOCYST DRAINAGE
- Strategy and mechanism for the prevention of hepatocellular carcinoma : Phosphorylated retinoid X receptor α is a critical target for hepatocellular carcinoma chemoprevention
- Pathological role of CD44 on NKT cells in carbon tetrachloride-mediated liver injury
- Abolishment of Pleural Effusion as the Initial Manifestation of Chronic Myelomonocytic Leukemia without Chemotherapy
- Significant effect of hepatitis C virus specific CTLs on viral clearance in patients with type C chronic hepatitis treated with antiviral agents
- Diffuse Large B-Cell Lymphoma with Tumor Thrombosis in the Portal Vein
- Prospective study on early virologic response to treatment with interferon α-2b plus ribavirin in patients with chronic hepatitis C genotype 1b
- Expression of survivin and of antigen detected by a novel monoclonal antibody, T332, is associated with outcome of diffuse large B-cell lymphoma and its subtypes
- Liver injury induced by lipopolysaccharide is mediated by TNFR-1 but not by TNFR-2 or Fas in mice
- Acute Myeloid Leukemia With t(11;19) (q23;pl3) Developing in an Adult T-Cell Leukemia Patient Treated With Combined Chemotherapy Including Etoposide
- HSC Activation and Retinoid Metabolism
- Indirect Calorimetry and Anthropometry to Estimate Energy Metabolism in Patients with Liver Cirrhosis
- (-)-Epigallocatechin gallate suppresses the growth of human hepatocellular carcinoma cells by inhibiting activation of the vascular endothelial growth factor-vascular endothelial growth factor receptor axis
- Clinical trial : rebamipide promotes gastric ulcer healing by proton pump inhibitor after endoscopic submucosal dissection-a randomized controlled study
- Guidelines for the treatment of chronic hepatitis and cirrhosis due to hepatitis C virus infection for the fiscal year 2008 in Japan
- Guidelines for the treatment of chronic hepatitis and cirrhosis due to hepatitis B virus infection for the fiscal year 2008 in Japan
- Insulin resistance raises the risk for recurrence of stage I hepatocellular carcinoma after curative radiofrequency ablation in hepatitis C virus-positive patients : A prospective, case series study
- Lymphoid Blast Crisis During Interferon-α Therapy in a Patient With Chronic Myelogenous Leukemia in Myeloid Blast Crisis
- Hydroxyurea-Induced Fever in a Patient With Chronic Myelogenous Leukemia
- Type II Citrullinemia in an Elderly Patient Treated with Living Related Partial Liver Transplantation
- Indirect Calorimetry and Anthropometry to Estimate Energy Metabolism in Patients with Liver Cirrhosis
- (肝臓領域)
- Cell Cycle-Dependent Priming Action of Granulocyte Colony-Stimulating Factor (G-CSF) Enhances in vitro Apoptosis Induction by Cytarabine and Etoposide in Leukemia Cell Lines
- Accumulation of refractory factors for pegylated interferon plus ribavirin therapy in older female patients with chronic hepatitis C
- Factors predictive of sustained virological response following 72 weeks of combination therapy for genotype 1b hepatitis C
- Cholangioscopic view of IgG4-related sclerosing cholangitis
- EUS-FNA for suspected malignant biliary strictures after negative endoscopic transpapillary brush cytology and forceps biopsy
- PREDICTIVE FACTORS FOR PAIN RELIEF AFTER ENDOSCOPIC ULTRASOUND-GUIDED CELIAC PLEXUS NEUROLYSIS
- Coexistence of inversion 16 and the Philadelphia chromosome comprising P190 BCR/ABL in chronic myeloid leukemia blast crisis
- Effect of Inadequate Fits of Superstructures on the Maintenance of Osseointegration
- Comparison of axial force and cell width of self-expandable metallic stents : which type of stent is better suited for hilar biliary strictures?
- First report of drug-induced esophagitis by deferasirox
- Supplemental Effect of 1,2-Dimethylhydrazine on Methylazoxymethanol Acetate-induced Colon Carcinogenesis in Rats; Effect of .ALPHA.-difluoromethylornithine on the Progression of Tumorigenesis.
- Purification and Partial Characterization of Rat Epididymal Retinoic Acid-binding Protein, and Its Immunohistochemical Localization.