In Vitro Development and Production of Offspring from Bovine Nuclear Transfer Embryos Cultured in a Serum-free Medium

スポンサーリンク

概要

• 論文の詳細を見る

• Newly developed serum-free media (IVD101 in the absence of bovine cumulus/granulosa cell (BCGC) coculture and IVMD101 in the presence of BCGC coculture) have recently been found to improve the yield and quality of bovine blastocysts from in vitro matured and fertilized oocytes [1]. The present study was undertaken with two objectives: first, to examine whether bovine nuclear transfer embryos could develop to the blastocyst stage when cultured in the serum-free media (IVD101 and IVMD101): second, to confirm the potency of the nuclear transfer blastocysts to develop to viable offspring after transfer to recipient cows. In vivo-derived embryos were used as donor nuclei and in vitro-derived oocytes were used as recipient oocytes for nuclear transfer. Recipient oocytes at the second metaphase were enucleated and the fusion of donor blastomere with the recipient oocytes was carried out by electrostimulation. The nuclear transfer embryos cultured in IVMD101 with BCGC coculture and IVD101 without BCGC coculture resulted in 20.4% and 27.5% blastocyst formation rate, respectively, which were similar to that (23.4%) of nuclear transfer embryos cultured in a conventional serum-supplemented medium (CR1aa+5% calf serum; CS) with BCGC coculture. Transfer of the nuclear transfer blastocysts developed in IVMD101, IVD101 and CR1aa+5%CS resulted in one pregnancy (1/7), two pregnancies (2/6), and two pregnancies (2/5), respectively, when examined on Day 40. The corresponding numbers of normal live calves born were 1, 1, and 1. Our results indicate that the two different serum-free media may be useful for the in vitro development of nuclear transfer embryos and the subsequent production of normal calves after transfer to recipient cows.

• 日本繁殖生物学会の論文
• 1999-04-01

著者

• HOSHI Hiroyoshi

  Research Institute for the Functional Peptides

• Aoyagi Kazushige

  Animal Experiment Department Yamagata Prefectural Agricultural Research&training Center

• Ito Hiroyasu

  Animal Experiment Department Yamagata Prefectural Agricultural Research&training Center

• SAITOU Maki

  Animal Experiment Department, Yamagata Prefectural Agricultural Research&Training Center

• KANAUCHI Tuneo

  Animal Experiment Department, Yamagata Prefectural Agricultural Research&Training Center

• KOBAYSHI Masato

  Animal Experiment Department, Yamagata Prefectural Agricultural Research&Training Center

• Saitou Maki

  Animal Experiment Department Yamagata Prefectural Agricultural Research&training Center

• Kanauchi Tuneo

  Animal Experiment Department Yamagata Prefectural Agricultural Research&training Center

• Kobayshi Masato

  Animal Experiment Department Yamagata Prefectural Agricultural Research&training Center

関連論文

• Successful Piglet Production in a Chemically Defined System for In-vitro Production of Porcine Embryos : Dibutyryl Cyclic AMP and Epidermal Growth Factor-family Peptides Support In-vitro Maturation of Oocytes in the Absence of Gonadotropins
• Blastocyst Production from In Vitro-produced Day-2 Bovine Embryos Classified by Cleavage Stage, and Cytogenetical Evaluation of the Resultant Day-8 Blastocysts
• Fatty Acid Composition of Bovine Embryos Cultured in Serum-Free and Serum-Containing Medium during Early Embryonic Development
• EFFICIENT PRODUCTION OF HIGH QUALITY BIOPSIED, CRYOPRESERVED BOVINE BLASTOCYSTS IN A SERUM-FREE MEDIUM
• DEVELOPMENT OF SCANNING ELECTROCHEMICAL MICROSCOPY TO MEASURE THE RESPIRATION OF MAMMALIAN EMBRYOS(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• In Vitro Culture and Evaluation of Embryos for Production of High Quality Bovine Embryos
• Expression of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) and Collagenase Genes in Preimplantation Bovine Embryos
• In Vitro Development and Production of Offspring from Bovine Nuclear Transfer Embryos Cultured in a Serum-free Medium
• Evaluation of Bovine Embryos Produced in High Performance Serum-Free Media
• Successful Production of Piglets Derived from Expanded Blastocysts Vitrified Using a Micro Volume Air Cooling Method without Direct Exposure to Liquid Nitrogen

スポンサーリンク