Evaluation of Bovine Embryos Produced in High Performance Serum-Free Media
スポンサーリンク
概要
- 論文の詳細を見る
This review evaluates the quality of bovine embryos developed from in vitro-matured (IVM) and -fertilized (IVF) oocytes cultured in either serum-free or serum-containing media. Bovine embryos cultured in serum-supplemented medium contain numerous cytoplasmic lipid droplets and immature mitochondoria compared to those cultured in serum-free medium. The accumulation of cytoplasmic lipids in embryos developed in serum-containing medium may be a result of incorporation of lipoproteins from the serum and may result in impaired function of mitochondria. The improved serum-free media (IVMD101 and IVD101) offer several advantages over culture in serum-containing medium, including increased rates of blastocyst formation and higher cell numbers. Additionally, the survival and hatching rates of embryos produced in serum-free media after post-thaw culture were superior to those of embryos produced in the serum-containing medium, suggesting that the abnormal accumulation of cytoplasmic lipids in embryos may have a negative effect on the sensitivity of embryos to chilling and freezing. These serum-free culture systems have proven to be beneficial for the production of good quality embryos from IVM-IVF bovine oocytes. Furthermore, recent studies have shown a correlation between mitochondrial function (oxygen consumption) and embryo quality. A new method using scanning electrochemical microscopy may be capable of asessing the viability and developmental potential of bovine embryos.
- 日本繁殖生物学会の論文
- 2003-06-01
著者
-
HOSHI Hiroyoshi
Research Institute for the Functional Peptides
-
Abe Hiroyuki
Research Institute For The Functional Peptides
-
Abe Hiroyuki
Research Institute For Scientific Measurements Tohoku University
関連論文
- Successful Piglet Production in a Chemically Defined System for In-vitro Production of Porcine Embryos : Dibutyryl Cyclic AMP and Epidermal Growth Factor-family Peptides Support In-vitro Maturation of Oocytes in the Absence of Gonadotropins
- Blastocyst Production from In Vitro-produced Day-2 Bovine Embryos Classified by Cleavage Stage, and Cytogenetical Evaluation of the Resultant Day-8 Blastocysts
- Electron Energy-Loss Spectroscopy Study of the Metal-Insulator Transition in (V_Cr_x)_2O_3 (x=0.012)
- Electron Energy-Loss Spectroscopy Study of the Metal-Insulator Transition in V_2O_3
- Fatty Acid Composition of Bovine Embryos Cultured in Serum-Free and Serum-Containing Medium during Early Embryonic Development
- Temperature Dependence of the Volume-plasmon Energy in Silver
- EFFICIENT PRODUCTION OF HIGH QUALITY BIOPSIED, CRYOPRESERVED BOVINE BLASTOCYSTS IN A SERUM-FREE MEDIUM
- Isolation of Antidiabetic Components from White-Skinned Sweet Potato (Ipomoea batatas L.)
- Antidiabetic Activity of White Skinned Sweet Potato (Ipomoea batatas L.) in Obese Zucker Fatty Rats
- DEVELOPMENT OF SCANNING ELECTROCHEMICAL MICROSCOPY TO MEASURE THE RESPIRATION OF MAMMALIAN EMBRYOS(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- In Vitro Culture and Evaluation of Embryos for Production of High Quality Bovine Embryos
- Expression of Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) and Collagenase Genes in Preimplantation Bovine Embryos
- In Vitro Development and Production of Offspring from Bovine Nuclear Transfer Embryos Cultured in a Serum-free Medium
- Evaluation of Bovine Embryos Produced in High Performance Serum-Free Media
- Performance Improvement of the Transmission High-resolution EELS Microscope and Application to Vanadium Oxides(Abstracts of Doctoral Dissertations,Annual Report (from April 1996 to March 1997))
- Electron Energy-loss Spectroscopy Study of the Metal-insulator Transition in VO 2
- Successful Production of Piglets Derived from Expanded Blastocysts Vitrified Using a Micro Volume Air Cooling Method without Direct Exposure to Liquid Nitrogen