Recombinant Bovine Growth Hormone Stimulates Nuclear Maturation of Bovine Oocytes in Vitro and Promotes Subsequent Embryonic Development

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概要

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• The present study was undertaken to examine the effects of recombinant bovine growth hormone (rbGH) on nuclear and cytoplasmic maturation of bovine oocytes in vitro and subsequent embryonic development after in vitro fertilization. When cumulus-oocyte complexes (COCs) and cumulus-free oocytes were cultured in modified Medium 199 supplemented with 0, 0.1, 1, 10, 100 or 1000 ng/ml rbGH for 24 h, no cumulus expansion was observed in most COCs in the presence of any of the tested concentrations of rbGH. The efficacy of nuclear maturation of oocytes to metaphase II was promoted by rbGH only in COCs showing higher proportions at 10-1000 ng/ml (77-78%) than at 0-1 ng/ml (57-64%). When COCs were cultured in the presence or absence of 10 ng/ml rbGH for 0-9 h, the proportions of oocytes that reached prometaphase I and metaphase I after 6 and 9 h of culture, respectively, were higher in the presence (53% and 51%, respectively) than in the absence (34% and 30%, respectively) of rbGH. After in vitro maturation and fertilization, no differences were observed in the penetration rate (82-91%), pronuclear formation (27-45%) and polyspermy (23-29%) at 8 h post-insemination among cumulus-enclosed oocytes cultured in the presence of different concentrations (0-1000 ng/ml) of rbGH. When starting 8 h after insemination, the oocytes were cultured for 136 h in a chemically defined medium, 21-25% of oocytes developed to the morula stage or beyond without difference among different concentrations of rbGH during oocyte maturation. However, at 192 h post-insemination higher proportions of oocytes developed to the blastocyst stage when COCs were matured in the presence (13-15%) than in the absence (6%) of 10-1000 ng/ml rbGH. These results indicate that a stimulatory effect of rbGH on bovine oocyte maturation is dependent on the cumulus cells. Presence of rbGH in the media improved maturation rate and subsequent embryonic development to the blastocyst stage.

• 日本繁殖生物学会の論文
• 1998-02-01

著者

• Niwa K

  Okayama Univ. Okayama Jpn

• NIWA Koji

  Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japa

• IGA Kosuke

  The Graduate School of Natural Science and Technology

• Iga Kosuke

  Reproductive Biology And Technology Research Subteam National Agricultural Research Center For Tohok

• Niwa Koji

  Division Of Animal Science And Technology Faculty Of Agriculture Okayama University

• Iga K

  National Inst. Animal Ind. Ibaraki Jpn

• IGA Kosuke

  Division of Animal Science and Technology, Faculty of Agriculture, Okayama University

• BARTKE Andrzej

  Department of Physiology, Southern Illinois University School of Medicine

• Bartke Andrzej

  Department Of Physiology Southern Illinois University School Of Medicine

• Iga K

  Okayama Univ. Okayama Jpn

関連論文

• Effects of Cysteine in Serum-Free Maturation Medium on Male Pronuclear Formation of Maturing Pig Oocytes Penetrated In Vitro
• 体外成熟豚卵子透明帯へのレクチンの結合と体外における精子の接着
• Growth of Bovine Oocyte-Granulosa Cell Complexes Cultured Individually in Microdrops of Various Sizes
• Effects of Cumulus Cells on the Ability of Pig Oocytes to Utilize Cysteine or Cystine During Maturation In Vitro
• Effects of Cumulus Cells on the Ability of Pig Oocytes to Utilize Cysteine or Cystine During Maturation In Vitro
• Effects of Cysteine in Serum-Free Maturation Medium on Male Pronuclear Formation of Maturing Pig Oocytes Penetrated In Vitro
• Factors Affecting Development In Vitro of Bovine and Rat 1-Cell Embryos
• Developmental Abilities of Rat and Mouse Pronuclear Eggs Following Interspecific Nuclear or Cytoplasmic Transplantation
• Possible Involvement of Insulin-like Growth Factor-I in Mediating the Stimulatory Effect of Recombinant Bovine Growth Hormone on Maturation of Bovine Oocytes In Vitro
• Recombinant Bovine Growth Hormone Stimulates Nuclear Maturation of Bovine Oocytes in Vitro and Promotes Subsequent Embryonic Development
• Requirement of Cysteine during in Vitro Maturation of Pig Oocytes for Male Pronuclear Formation
• 透明帯との相互作用に関与する凍結-融解豚射出精子におけるグリコシダーゼの同定と局在性
• Effects of Follicle Cells on in vitro Penetration of Pig Oocytes by Cryopreserved, Ejaculated Spermatozoa
• Optimization of Oxygen Concentration for Growing Bovine Oocytes In Vitro : Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes
• Milrinone Treatment of Bovine Oocytes During In Vitro Maturation Benefits Production of Nuclear Transfer Embryos by Improving Enucleation Rate and Developmental Competence
• Optimization of Oxygen Concentration for Growing Bovine Oocytes In Vitro: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes
• Effect of the Presence of Glucose during Fertilization and/or Culture in a Chemically Semi-Defined Medium on the Development of Bovine Oocytes Matured and Fertilized in Vitro.

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