Effects of Cysteine in Serum-Free Maturation Medium on Male Pronuclear Formation of Maturing Pig Oocytes Penetrated In Vitro
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概要
- 論文の詳細を見る
Pig immature oocytes were cultured for 24, 36 and 48 h in serum-free maturation medium with or without 0.57 mM cysteine. The addition of cysteine to the medium was associated with increased glutathione synthesis by oocytes but did not promote nor inhibit nuclear maturation, sperm penetration in vitro, and decondensation of sperm nuclei in penetrated oocytes. The incidence of activation of penetrated oocytes 14 h after insemination in vitro was lower in those cultured both in the presence and absence of cysteine for 24 h than 36 and 48 h. The lower ability of oocytes cultured for 24 h to be activated was not improved by neither the addition of cysteine (0.57 mM) in fertilization medium nor prolonged culture time after insemination. However, male pronuclear formation in activated oocytes after sperm penetration at any stages of maturation was largely accelerated when cysteine was added to maturation medium. An increased concentration of glutathione may induce full decondensation of sperm nuclei in immature pig oocytes penetrated in vitro ensuring transformation of the decondensed sperm nuclei to male pronuclei only in synchronization with oocyte activation.
- 日本繁殖生物学会の論文
- 1997-02-01
著者
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Sawai Ken
Hokkaido Animal Research Center
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Sawai Ken
Division Of Animal Science And Technology Faculty Of Agriculture Okayama University
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Sawai Ken
Faculty Of Agriculture Iwate University
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Niwa K
Okayama Univ. Okayama Jpn
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Funahashi Hiroaki
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japa
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Wang Wei-Hua
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japa
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NIWA Koji
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japa
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Funahashi Hiroaki
Division Of Animal Science And Technology Faculty Of Agriculture Okayama University
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Niwa Koji
Division Of Animal Science And Technology Faculty Of Agriculture Okayama University
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Sawai Ken
岩手大学 農学部
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Wang Wei-hua
Division Of Animal Science And Technology Faculty Of Agriculture Okayama University
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