Possible Involvement of Insulin-like Growth Factor-I in Mediating the Stimulatory Effect of Recombinant Bovine Growth Hormone on Maturation of Bovine Oocytes In Vitro

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概要

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• The present study was undertaken to determine whether insulin-like growth factor-I (IGF-I) is involved in mediating the stimulatory effects of growth hormone (GH) on nuclear maturation of bovine oocytes in vitro and subsequent embryonic development after in vitro fertilization. When cumulus-oocyte complexes (COCs) or cumulus-free oocytes were cultured in modified Medium 199 supplemented with 0-100 ng/ml recombinant human IGF-I (rhIGF-I), the proportion of COCs with slight cumulus expansion was increased with increasing concentration of rhIGF-I after 12 and 24 h of culture. Nuclear maturation of oocytes to the metaphase II (M-II) stage was promoted by rhIGF-I only in COCs, with higher proportions of M-II oocytes at 10-100 ng/ml (74 ± 3-76 ± 3%) than at 0-1 ng/ml (53 ± 4-61 ± 1%) after 24 h of culture. When COCs were cultured in the presence of 100 ng/ml rhIGF-I or 10 ng/ml recombinant bovine GH (rbGH) and/or anti-IGF-I antibody (Ab) for 24 h, the proportion of oocytes reaching the M-II stage was higher with rhIGF-I (73 ± 2%) or rbGH (74 ± 1%) than in the absence of these additives (54 ± 3%), in Ab alone (54 ± 3%), or with IGF-I or GH in combination with Ab (54 ± 3-58 ± 1%). The inhibition of the stimulatory effects of rbGH on nuclear maturation by Ab was dose-dependent. After in vitro maturation and fertilization, no differences were observed in the penetration rate (75 ± 3-77 ± 6%), pronuclear formation (56 ± 3-64 ± 10%) and polyspermy (28 ± 4-36 ± 5%) at 8 h post-insemination among cumulus enclosed oocytes cultured in the presence of different additives. When the oocytes were cultured in a chemically defined medium starting from 8 h after insemination, 20 ± 1-26 ± 2% of oocytes developed to the ≥ morula stage 144 h post-insemination without difference among different additives during maturation. However, at 192 h post-insemination, higher proportions of oocytes developed to the blastocyst stage when COCs were matured in the presence of rhIGF-I (16 ± 1%) or rbGH (15 ± 2%) than in the absence of the additives (4 ± 2%) or when IGF-I or GH was combined with Ab (4 ± 2-5 ± 3%). These results suggest that the stimulatory effects of GH on bovine oocyte maturation may not be direct, but mediated via IGF-I probably produced by the cumulus cells.

• 日本繁殖生物学会の論文
• 1998-08-01

著者

• Niwa K

  Okayama Univ. Okayama Jpn

• NIWA Koji

  Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japa

• IGA Kosuke

  The Graduate School of Natural Science and Technology

• Iga Kosuke

  Reproductive Biology And Technology Research Subteam National Agricultural Research Center For Tohok

• Niwa Koji

  Division Of Animal Science And Technology Faculty Of Agriculture Okayama University

• Iga K

  National Inst. Animal Ind. Ibaraki Jpn

• IGA Kosuke

  Division of Animal Science and Technology, Faculty of Agriculture, Okayama University

• BARTKE Andrzej

  Department of Physiology, Southern Illinois University School of Medicine

• Bartke Andrzej

  Department Of Physiology Southern Illinois University School Of Medicine

• Iga K

  Okayama Univ. Okayama Jpn

関連論文

• Effects of Cysteine in Serum-Free Maturation Medium on Male Pronuclear Formation of Maturing Pig Oocytes Penetrated In Vitro
• 体外成熟豚卵子透明帯へのレクチンの結合と体外における精子の接着
• Growth of Bovine Oocyte-Granulosa Cell Complexes Cultured Individually in Microdrops of Various Sizes
• Effects of Cumulus Cells on the Ability of Pig Oocytes to Utilize Cysteine or Cystine During Maturation In Vitro
• Effects of Cumulus Cells on the Ability of Pig Oocytes to Utilize Cysteine or Cystine During Maturation In Vitro
• Effects of Cysteine in Serum-Free Maturation Medium on Male Pronuclear Formation of Maturing Pig Oocytes Penetrated In Vitro
• Factors Affecting Development In Vitro of Bovine and Rat 1-Cell Embryos
• Developmental Abilities of Rat and Mouse Pronuclear Eggs Following Interspecific Nuclear or Cytoplasmic Transplantation
• Possible Involvement of Insulin-like Growth Factor-I in Mediating the Stimulatory Effect of Recombinant Bovine Growth Hormone on Maturation of Bovine Oocytes In Vitro
• Recombinant Bovine Growth Hormone Stimulates Nuclear Maturation of Bovine Oocytes in Vitro and Promotes Subsequent Embryonic Development
• Requirement of Cysteine during in Vitro Maturation of Pig Oocytes for Male Pronuclear Formation
• 透明帯との相互作用に関与する凍結-融解豚射出精子におけるグリコシダーゼの同定と局在性
• Effects of Follicle Cells on in vitro Penetration of Pig Oocytes by Cryopreserved, Ejaculated Spermatozoa
• Optimization of Oxygen Concentration for Growing Bovine Oocytes In Vitro : Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes
• Milrinone Treatment of Bovine Oocytes During In Vitro Maturation Benefits Production of Nuclear Transfer Embryos by Improving Enucleation Rate and Developmental Competence
• Optimization of Oxygen Concentration for Growing Bovine Oocytes In Vitro: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes
• Effect of the Presence of Glucose during Fertilization and/or Culture in a Chemically Semi-Defined Medium on the Development of Bovine Oocytes Matured and Fertilized in Vitro.

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