Reversible Inactivation of an Intracellular Uricase from Bacillus fastidiosus via Dissociation of Homotetramer into Homodimers in Solutions of Low Ionic Strength
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概要
- 論文の詳細を見る
An intracellular uricase from Bacillus fastidiosus with high catalytic capacity and strong resistance to xanthine was inactivated in water but could be essentially re-activated in solutions of high ionic strength. By polyacrylamide gel electrophoresis (PAGE), gradient PAGE, sodium-dodecyl-sulfate-PAGE, gel-filtration through Sephadex G200, and activity staining with peroxidase and its chromatogenic substrate, this homotetrameric uricase in water was found to dissociate into inactive homodimers that could form active homotetramers again in solutions of high ionic strength. Sensitivity to low ionic strength of solutions complicates formulation of this uricase as a drug and its elimination requires protein engineering.
- 社団法人 日本農芸化学会の論文
- 2009-09-23
著者
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Zhang Chun
Unit For Biotransformation And Protein Biotechnology Chongqing Key Laboratory Of Biochemical And Mol
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Liao Fei
Unit For Biotransformation And Protein Biotechnology Chongqing Medical University
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ZHAO Yunsheng
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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YANG Xiaolan
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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LI Xiaoyan
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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BU Youquan
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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DENG Ping
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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ZHANG Chun
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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FENG Juan
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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XIE Yanling
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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ZHU Sha
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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YUAN Huidong
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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LIAO Fei
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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Yu Ming'an
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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Li Xiang
Unit For Biotransformation And Protein Biotechnology Chongqing Key Laboratory Of Biochemical And Mol
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Feng Juan
Unit For Biotransformation And Protein Biotechnology Chongqing Medical University
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Yang Xiaolan
Unit For Biotransformation And Protein Biotechnology Chongqing Medical University
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Zhao Yunsheng
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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Zhu Sha
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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Bu Youquan
Unit For Biotransformation And Protein Biotechnology Chongqing Key Laboratory Of Biochemical And Mol
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Yuan Huidong
Unit For Biotransformation And Protein Biotechnology Chongqing Medical University
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Deng Ping
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
関連論文
- Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus
- Effects of Modification of Amino Groups with Poly (Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus
- Reversible Inactivation of an Intracellular Uricase from Bacillus fastidiosus via Dissociation of Homotetramer into Homodimers in Solutions of Low Ionic Strength
- Kinetic Analysis of the Lactate-dehydrogenase-coupled Reaction Process and Measurement of Alanine Transaminase by an Integration Strategy
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