Soluble Expression in Escherichia coli of Active Human Cyclic Nucleotide Phosphodiesterase Isoform 4B2 in Fusion with Maltose-Binding Protein
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概要
- 論文の詳細を見る
Recombinant expression in Escherichia coli of human cyclic nucleotide phosphodiesterase 4B2 (hPDE4B2) fused to maltose-binding-protein (MBP-hPDE4B2) was investigated. hPDE4B2 DNA amplified via nested RT-PCR with total RNAs from U937 cells was ligated with pMAL-p2x. After induction at 18 °C for 16 h, soluble MBP-hPDE4B2 was produced in E. coli. MBP-hPDE4B2 after amylose-resin chromatography showed 35% homogeneity, and its Michaelis-Menten constant was 10±2 μM (n=3). Rolipram had a dissociation constant of 9±2 nM (n=2), and zinc ion was a potent inhibitor. Hence, MBP-hPDE4B2 was expressed in E. coli as a soluble active protein.
- 社団法人 日本農芸化学会の論文
- 2009-04-23
著者
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Liu Yin
Unit For Biotransformation And Protein Biotechnology Chongqing Medical University
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Liao Fei
Unit For Biotransformation And Protein Biotechnology Chongqing Medical University
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YANG Xiaolan
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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DENG Ping
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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XIE Yanling
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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ZHU Sha
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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LIAO Fei
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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Yu Ming'an
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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Li Xiang
Unit For Biotransformation And Protein Biotechnology Chongqing Key Laboratory Of Biochemical And Mol
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Yang Xiaolan
Unit For Biotransformation And Protein Biotechnology Chongqing Medical University
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Zhao Yunsheng
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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LI Zhirong
Unit for Biotransformation and Protein Biotechnology, Chongqing Medical University
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Zhu Sha
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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Li Zhirong
Unit For Biotransformation And Protein Biotechnology Chongqing Medical University
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Deng Ping
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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YANG Genqing
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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LI Xiang
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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GAN Zhiyong
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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LIU Yin
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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LI Zhirong
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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LIAO Juan
Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, Chongqing Medical University
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Liao Juan
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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Gan Zhiyong
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
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Yang Genqing
Chongqing Key Laboratory Of Biochemical And Molecular Pharmacology Chongqing Medical University
関連論文
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- Reversible Inactivation of an Intracellular Uricase from Bacillus fastidiosus via Dissociation of Homotetramer into Homodimers in Solutions of Low Ionic Strength
- Kinetic Analysis of the Lactate-dehydrogenase-coupled Reaction Process and Measurement of Alanine Transaminase by an Integration Strategy
- Soluble Expression in Escherichia coli of Active Human Cyclic Nucleotide Phosphodiesterase Isoform 4B2 in Fusion with Maltose-Binding Protein