Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus
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概要
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After modification with monomethoxyl-poly(ethylene glycol)-5000, a recombinant intracellular uricase from Bacillus fastidiosus ATCC 29604 showed residual activity of about 65%, a thermo-inactivation half-life >85 h, a circulating half-life about 20 h in rats in vivo, consistent effects of common cations, and consistent optima for reaction temperature and pH. Thus, this uricase can be formulated via modification with monomethoxyl-poly(ethylene glycol).
著者
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Zhang Chun
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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Yang Xiaolan
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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Feng Juan
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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YUAN Yonghua
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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LI Xiang
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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BU Youquan
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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XIE Yanling
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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YUAN Huidong
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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LIAO Fei
Unit for Biotransformation and Protein Biotechnology, Chongqing Key Laboratory of Biochemical and Mo
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Zhang Chun
Unit For Biotransformation And Protein Biotechnology Chongqing Key Laboratory Of Biochemical And Mol
関連論文
- Effects of Modification of Amino Groups with Poly(Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus
- Effects of Modification of Amino Groups with Poly (Ethylene Glycol) on a Recombinant Uricase from Bacillus fastidiosus
- Reversible Inactivation of an Intracellular Uricase from Bacillus fastidiosus via Dissociation of Homotetramer into Homodimers in Solutions of Low Ionic Strength
- Kinetic Analysis of the Lactate-dehydrogenase-coupled Reaction Process and Measurement of Alanine Transaminase by an Integration Strategy