A New Assay Based on Fluorescence Resonance Energy Transfer to Determine the Binding Affinity of Bcl-X_L Inhibitors
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概要
- 論文の詳細を見る
We developed a new assay of Bcl-xL inhibitors based on fluorescence resonance energy transfer that occurs between an AEDANS-labeled Bak-BH3 peptide and three tryptophans in the BH1 and BH2 domains of Bcl-xL. The method can tolerate up to 5% DMSO, and it was validated with several Bcl-xL inhibitors. It can be adapted to screen for compounds targeting other Bcl-2 family proteins.
- 社団法人 日本農芸化学会の論文
- 2008-07-23
著者
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SHEN Xu
Department of Chemistry, Tongji University
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Shen Xu
Drug Discovery And Design Center State Key Laboratory Of Drug Research Shanghai Institute Of Materia
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Shen Xu
Department Of Chemistry Tongji University
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Jiang Hualiang
Drug Discovery And Design Center State Key Laboratory Of Drug Research Shanghai Institute Of Materia
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Feng Yu
Department Of Molecular Pharmacology State Key Laboratory Of Drug Research Shanghai Institute Of Mat
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CHEN Kaixian
Center for Drug Design and Discovery, State Key Laboratory of Drug Research, Shanghai Institute of M
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JIANG Hualiang
Center for Drug Design and Discovery, State Key Laboratory of Drug Research, Shanghai Institute of M
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LIU Dongxiang
Department of Molecular Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Scien
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Chen Kaixian
Center For Drug Design And Discovery State Key Laboratory Of Drug Research Shanghai Institute Of Mat
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Liu Dongxiang
Department Of Molecular Pharmacology State Key Laboratory Of Drug Research Shanghai Institute Of Mat
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FENG Yu
Department of Chemistry and Ecological Engineering, Guangxi University for Nationalities
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