Bactericidal Effect of HM-242, a Novel Disinfectant, against Pseudomonas aeruginosa

スポンサーリンク

概要

• 論文の詳細を見る

• The bactericidal effect of HM-242, a novel antimicrobial agent, against Pseudomonas aeruginosa was investigated by using Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values and the time-kill study. Furthermore, we also morphologically investigated its effect against P. aeruginosa by use of transmission electron microscopy (TEM) in comparison with that of chlorhexidine digluconate (CHG). The bactericidal activity of HM-242 after 1 min incubation evaluated by MBC was 25 μg/mL, while the MBC of CHG was 50 μg/mL. In the time-kill study, the killing activity of HM-242 with 25 μg/mL incubation was stronger than that of CHG with 50 μg/mL incubation. MIC values of HM-242 and CHG against P. aeruginosa were 25 μg/mL and 12.5 μg/mL, respectively. We also observed via TEM the morphological changes in the test bacteria after being treated with each drug at 1/2MBC, 1MBC, 2MBC and 4MBC after 1 min or 5 min incubation. Under treatment with the same concentration of the test drugs, the cell damage with HM-242 treatment was greater than that with CHG. The appearance of empty cells was recognized at the concentrations greater than 50 μg/mL (2MBC) of HM-242 and 200 μg/mL of CHG (4MBC) after 1 min exposure, although no cell damage was evident below these concentrations. The cell-damaging effect against the test strain was dependent on the drug concentration and incubation time. The release of cell components and bleb formation were also recognized. These results suggest that HM-242 has more potent bactericidal activity in low concentrations under shorter time treatments than CHG. Both HM-242 and CHG act on the cell membrane and cell wall of P. aeruginosa and can destroy the cell integrity. We finally emphasize that HM-242 as well as CHG might be a suitable disinfectant for use in the medical field.

• 2010-03-10

著者

• SAKAGAMI YOSHIKAZU

  School of Agriculture, Kinki University

• Sakagami Yoshikazu

  School Of Agriculture Kinki University

• Maeda Takuya

  Hyogo University Of Health Sciences

• OKUNISHI Junji

  Central Research Laboratories, Maruishi Pharmaceutical Co., Ltd.

• Okunishi Junji

  Central Research Laboratories Maruishi Pharmaceutical Co. Ltd.

• NISHIMURA HIROSHI

  Osaka Prefectural Institute of Public Health

• TAKADA AKIRA

  School of Agriculture, Kinki University

• INADA TAKAE

  School of Agriculture, Kinki University

• MAEDA SHIROU

  Hamari Chemicals Ltd.

• NISHIHARA TSUTOMU

  Hyogo University of Health Sciences

• KOMEMUSHI SADAO

  Graduate School of Osaka City University

• Takada Akira

  School Of Agriculture Kinki University

• Nishihara Tsutomu

  Hyogo Univ. Health Sci. Kobe Jpn

• Inada Takae

  School Of Agriculture Kinki University

• Maeda Takuya

  Hyogo Ion Beam Medical Center (HIBMC), Tatsuno, Hyogo 679-5165, Japan

関連論文

• Phylogenetic Analysis of Carotenoid-Producing Marine Microorganisms Around the Coral Reefs of the Kerama Islands of Okinawa
• Bactericidal Effect of HM-242, a Novel Disinfectant, against Pseudomonas aeruginosa
• Cleavage Specificity of Coxsackievirus 3C Proteinase for Peptide Substrate (2):Importance of the P2 and P4 Residues
• Cleavage Specificity of Coxsackievirus 3C Proteinase for Peptide Substrate
• Reduction of HIV-1 Infectivity and Reverse Transcriptase Activity by High Hydrostatic Pressure Treatment
• Anti-VRE and Anti-MRSA Activities of New Quinolones and Their Synergism with Commercial Antibiotics. Part 2
• Discovery of Anti-Infective Drugs Using Genoma-Based Technologies
• Flavonoid Glycosides, α-Glucosidase Inhibitors, from Leaves of Zizyphus jujuba MILL. var. inermis REHD.(Natural Medicine Note)
• Measurement of DNA Double-Strand Break Yield in Human Cancer Cells by High-Current, Short-Duration Bunches of Laser-Accelerated Protons

スポンサーリンク