土壌中における Ralstonia solanacearum の菌体外多糖非生産株の誘発と同変異株の発病抑制効果
スポンサーリンク
概要
- 論文の詳細を見る
To suppress bacterial wilt of tomato caused by Ralstonia solanacearum with soil administration, soil conditions that influence the production of extracellular polysaccharides (EPS) in R. solanacearum were surveyed, and the prevention of this disease by strains that do not produce EPS (EPS- strains) was investigated. The culture conditions, using either liquid medium or sterilized soil were investigated to induce the phenotype conversion (PC) in relation to the reduction or loss of EPS production in R. solanacearum. EPS- colonies were frequently detected under unfavorable culture conditions, such the use of a rather alkaline medium (pH8 and pH8.5), higher temperature (37℃) or still culture rather than shaking culture. The culture conditions under which the EPS- colonies were detected with a high frequency varied depending on the strains tested. In the case of culture conditions of the bacterium using either sterilized of non-sterilized soil, EPS- colonies were also observed with a high frequency in all the cases, including, acidic soil, higher temperature, and soil moisture exceeding the maximum water holding capacity. Disease severity was evaluated after the EPS-producing strains were inoculated either alone or simultaneously with EPS-non-producing strains by mixing into soil where subsequently tomato was planted. When EPS-producing strains and EPS-non-producing strains were simultaneously inoculated, the damage was less serious than in the case of inoculation with EPS-producing strains alone. When the ratio of EPS-non-producing cells to EPS-producing cells increased, the effect on disease suppression was more pronounced. If the phenotype conversion of R. solanacearum living in soil could be induced with a high frequency by soil administration, bacterial wilt disease may be effectively suppressed.
- 日本土壌微生物学会の論文
- 2002-04-01
著者
関連論文
- 雲仙普賢岳水無川本流の乾式航空緑化工における施工10年後の土壌の肥沃度及び植生定着(第37回大会)
- 桜島荒廃地の緑化(第35回大会)
- 7-7 トマト青枯病抑止土壌における病原細菌の増殖抑制部位(7.土壌病害)
- 過酸化水素処理した土壌中のバイオマス量および稲わら分解
- 土壌微生物に及ぼす土壌侵食防止・緑化被覆シートの影響
- 各種温度でのススキの燃焼過程における元素組成と腐植組成の変化
- 土壌微生物バイオマス由来の可溶性C・N量におよぼす鉄およびアルミニウム水酸化物の影響 : 微生物バイオマスにおよぼす火山灰土壌中の鉄およびアルミニウム酸化物の影響(第1報)
- 22-3 土壌侵食防止・緑化被覆資材に関する研究(6) : 雲仙普賢岳における現地試験結果(2)(22.緑化技術)
- 土壌中の微生物バイオマス量と理化学性の関係 : 火山灰土壌と非火山灰土壌の比較
- 22-2 土壌侵食防止・緑化被覆資材に関する研究(5) : 雲仙普賢岳における現地試験結果(22.緑化技術)