マトリックス支援レーザー脱離イオン化飛行時間型質量分析計による使用済みコンタクトレンズケースから回収された微生物の迅速分類

概要

論文の詳細を見る
As an alternative to commonly used biochemical, morphological, or gene technological analysis, we have evaluated a novel method for rapid classification of microorganism retrieved from used contact lens cases based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Test microorganisms were isolated from contact lens cases. The cases were used daily for lens storage and care for 2 weeks. For this evaluation study, 659 microorganism isolates from the contact lens cases of 16 subjects were analyzed by MALDI-TOF-MS. The mass spectra obtained from the test isolates were compared with authentic spectra of a library database using application software Biotyper 2.0 (Bruker Daltonics). Results show that 644 of 659 (97.7%) isolates were successfully identified and classified into species by the MALDI-based method. Furthermore, these results were interpreted in terms of the risk of infectious disease. Our method is especially useful for simultaneous classification of a large number of test microorganisms because of fewer preparative steps and lower cost.
日本質量分析学会の論文
2009-08-01