Detection of an antibody to avian influenza virus by an electrochemical immunoassay (eELISA)
スポンサーリンク
概要
- 論文の詳細を見る
The detection of an antibody against nucleoprotein (NP) of the avian influenza virus (AIV) was attempted by an electrochemical immunoassay by combining a secondary antibody-alkaline phosphatase conjugate and 4-aminophenylphosphate. Released 4-aminophenol was quantitated electrochemically, and the current derived from oxidation of the hydrolysis product increased linearly over a wide primary antibody concentration range (10 - 1000 ng/ml). The detection limit of this electrochemical immunoassay for the antibody against the NP of AIV was found to be 10 ng/ml.
- 社団法人日本分析化学会の論文
- 2008-12-10
著者
-
Morimoto Kimio
Hano Manufacturing Co. Ltd.
-
TAKENAKA Shigeori
Department of Chemical System and Engineering, Kyusyu University
-
OHTSUKA Keiichi
Department of Applied Chemistry, Kyushu Institute of Technology
-
ENDO Hiroshi
Hano Manufacturing Co. Ltd.
-
VUONG Bui
Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary
-
OGAWA Haruko
Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary
-
IMAI Kunitoshi
Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary
-
Takenaka Shigeori
Department Of Applied Chemistry Faculty Of Engineering Kyushu University
-
Takenaka Shigeori
Department Of Applied Chemistry Kyushu Institute Of Technology
-
Ogawa Haruko
Research Center For Animal Hygiene And Food Safety Obihiro University Of Agriculture And Veterinary
-
Vuong Bui
Research Center For Animal Hygiene And Food Safety Obihiro University Of Agriculture And Veterinary
-
Imai Kunitoshi
Research Center For Animal Hygiene And Food Safety Obihiro University Of Agriculture And Veterinary
-
Ohtsuka Keiichi
Department Of Applied Chemistry Kyushu Institute Of Technology
-
Takenaka Shigeori
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
関連論文
- ECA (Electrochemical array) チップを用いたRAS変異解析(第105回日本外科学会定期学術集会)
- 水中におけるDNA-フラーレン会合体の形成(ナノ空間と分析化学)
- Photoisomerizable DNA Ligands. Spectral and Electrochemical Properties and Base-Pair Selectivity of Binding of Bis[2-(1-alkylpyridinium-4-yl)vinyl]benzen Dyes
- 「2009分析展」見聞記
- DNA二本鎖形成の電気化学検出において二本鎖DNAにインターカレートしたフェロセニルナフタレンジイミドの電気化学解析
- 簡易診断を目指した次世代チップの開発 (特集 次代をつくる実用研究開発)
- DNA四本鎖構造を利用したカリウムイオンの検出 (特集 強相関ソフトマテリアルの動的制御(Part1)) -- (第2編 水素結合を利用した階層構造の構築と機能化)
- Threading Intercalators as a New DNA Structural Probe
- バイオチップを利用した分析法-DNAチップを中心に-
- Detection of an antibody to avian influenza virus by an electrochemical immunoassay (eELISA)
- Genotyping of the Human Lipoprotein Lipase Gene by Ferrocenylnaphthalene Diimide-based Electrochemical Hybridization Assay
- Direct Detection of Single Nucleotide Polymorphism (SNP) with Genomic DNA by the Ferrocenylnaphthalene Diimide-based Electrochemical Hybridization Assay (FND-EHA)
- Electrochemical Detection of Base Pair Mutation
- フェロセン化ナフタレンジイミド誘導体の電気化学とそのヘアピンDNA修飾電極での挙動
- Enhanced Electron Transfer from Glucose Oxidase to DNA-Immobilized Electrode Aided by Ferrocenyl Naphthalene Diimide, a Threading Intercalator
- Invariant Vα14 Natural Killer T Cell Activation by Edible Mushroom Acidic Glycosphingolipids
- Surveillance of Avian Influenza Virus in Migratory Water Birds in Eastern Hokkaido, Japan
- Ferrocenylnaphthalene Diimide-based Electrochemical Ribonuclease Assay
- Development of the Gene Diagnostic Instrument for Clinical Use
- Analysis of the Complex of Oligonucleotide Duplexes with Ligands by MALDI-TOF Mass Spectroscopy
- Photo - induced DNA Cleavage by Water - soluble Cationic Fullerene Derivatives
- Study of the DNA Interaction with Water - soluble Cationic Fullerene Derivatives
- DNA - Controlled Photosomerization of Bis(2 - (1 - methyl - pyridinium - 4 - yl)vinyl) benzene
- Development of Electrochemical Protease Assay
- Direct Modification of mRNA by Ferrocenyl Carbodiimide and Its Application to Electrochemical Detection of mRNA
- Interaction Analysis of the Carcinoembryonic Antigen (CEA) with Its Monoclonal Antibody Immobilized on a Gold Surface Using Fourier Transform Infrared Reflection-Absorption Spectroscopy (FT-IR RAS)
- Electrochemical Detection of a Disease-specific Isoform of the Prion Protein (PrP^)
- Analysis of the interaction between trypsin and sunflower trypsin inhibitor (SFTI-1) immobilized on the gold surface
- Immobilization of a peptide probe by a peptide-nucleotide conjugate (PNC)
- Detection of cRNA Hybridized on a DNA Chip Using a Tetrakis-Acridinyl Peptide Cassette, Consisting of TAP and Partially Self-complementary Oligonucleotide, d[A_(TA)_]
- Development of a Gene Detection Assay Based on Photo-Induced Electron Transfer
- Design and Conformational Analysis of a Helical Peptide Based on the Amino Acid Propensity in the Secondary Structure of Globular Proteins
- Light-Induced DNA Cleavage by Bis-9-acridinyl Viologen Derivative
- Preparation of Carbodiimide-terminated Dithiolane Self-Assembly Monolayers as a New DNA-Immobilization Method
- Gene Detection Based on the Tetrakis-acridinyl Peptide (TAP) Cassette
- Binding mode of a bis-acridine orange peptide with double stranded DNA
- Synthesis of New Peptide Intercalators as a Future Fluorometric Visualization Reagent for DNA Microarray
- Design and Conformational Analysis of Helical Model Peptides Based on the Amino Acid Propensity in the Helix Boundary Regions of Globular Proteins
- Spontaneous Formation of a Molecular Net Assembly by Using Nucleotide Complementarity
- Highly Sensitive Probe for Gene Analysis By Electrochemical Approach
- Pseudo-polyferrocene Coating of Double Stranded DNA with Ferrocenylnaphthalene Diimide and Its Application for Electrochemical Gene Detection
- Electrochemical Detection of Periodontal Disease Using Protease Assay
- Structural Optimization of a DNA-Peptide Conjugate Aiming at Potassium Ion Sensing Agent in Living Cells
- Fluorescence Detection of Potassium Ion Using the G-Quadruplex Structure
- Electrochemical DNA Detection Using Supramolecular Interactions
- Lung Cytokine Gene Expression is Correlated with Increased Severity of Disease in a Novel H4N8 Influenza Virus Isolated from Shorebirds
- Analysis of Electrochemical Reaction of Ferrocenylnaphthalene Diimide Captured by Double-Stranded DNA during the Electrochemical Detection of DNA Hybridization