A New binding Assay of von Willebrand Factor and Glycoprotein Ib Using Solid-Phase Biotinylated Platelets
スポンサーリンク
概要
- 論文の詳細を見る
To obtain compounds that inhibit the interaction of von Willebrand factor (vWF) and glycoprotein (GP) Ib, a novel binding assay was established. The binding of fixed platelets to vWF-R497 mutant was quantified by a solid phase assay. In this assay, fixed platelets bound to the vWF-R497 mutant, carrying the deletion of Glu497-Tyr508 and the missense mutation of Arg545 to Ala, without binding modulators such as ristocetin. The Kd value of the binding was 2.8 nM, which was consistent with the result from liquid binding assay. The binding was inhibited by aurin tricarboxylic acid (ATA) and an anti GPIb antibody, AK2. Using this binding assay, we screened our library compounds and obtained D74-3736. This compound also inhibited ristocetin-induced platelet aggregation in the human platelet-rich plasma.
- 2008-10-20
著者
-
HAYATA Kenji
R&D Division, Exploratory Research Laboratories II, Daiichi-Sankyo Co., Ltd.
-
NAKAYAMA Takayuki
Department of Hematology and Oncology, Nagoya University Graduate School of Medicine
-
MATSUSHITA Tadashi
Department of Hematology and Oncology, Nagoya University Graduate School of Medicine
-
SAKANO Katsuichi
R&D Division, Exploratory Research Laboratories I, Daiichi-Sankyo Co., Ltd.
-
Hayata Kenji
R&d Division Exploratory Research Laboratories Ii Daiichi-sankyo Co. Ltd.
-
Nakayama Takayuki
Department Of Hematology And Oncology Nagoya University Graduate School Of Medicine
-
Nakayama Takayuki
Department Of Biological Sciences Graduate School Of Science And Technology Kumamoto University
-
Matsushita Tadashi
Department Of Hematology And Oncology Nagoya University Graduate School Of Medicine
-
Matsushita Tadashi
Department Of Applied Physics Faculty Of Engineering Nagoya University
-
Sakano Katsuichi
R&d Division Exploratory Research Laboratories I Daiichi-sankyo Co. Ltd.
関連論文
- A New binding Assay of von Willebrand Factor and Glycoprotein Ib Using Solid-Phase Biotinylated Platelets
- Definite diagnosis in Japanese patients with protein C deficiency by identification of causative PROC mutations
- Mutational analysis of SOS1 gene in acute myeloid leukemia
- Identification of amino acid residues responsible for von Willebrand factor binding to sulfatide by charged-to-alanine-scanning mutagenesis
- Recurrent Mutations of Factor XI Gene in Japanese
- Recurrent Intramural Hematoma of the Small Intestine in a Severe Hemophilia A Patient with a High Titer of Factor VIII Inhibitor : A Case Report and Review of the Literature
- High Titer of ADAMTS13 Inhibitor Associated with Thrombotic Microangiopathy of the Gut and Skeletal Muscle after Allogeneic Hematopoietic Stem Cell Transplantation
- Irrespective of CD34 expression, lineage-committed cell fraction reconstitutes and re-establishes transformed Philadelphia chromosome-positive leukemia in NOD/SCID/IL-2Rγc^ mice
- Establishment of New Highly Insulin-Sensitive Cell Lines and Screening of Compounds to Facilitate Glucose Consumption
- Classic Polyarteritis Nodosa Presenting Rare Clinical Manifestations in a Patient with Hemophilia A
- Identification of the regulatory elements of the human von Willebrand factor for binding to platelet GPIb. Importance of structural integrity of the regions flanked by the CYS1272-CYS1458 disulfide bond
- The Effects of DNA Synthesis Inhibitors Cell Cycle Synchronization in Cucumber (Cucumis sativus L.) Root Meristems
- Observations on Equal-Inclination Interference Fringes from a Thin Crystal of Silicon Using Nearly Parallel X-Ray Beam
- A Generalized Phase Space Optical Analysis of X-Ray Optical Systems Using Crystal Monochromators : REFINED SPECTROSCOPY, X-RAY OPTICS AND INSTRUMENTATION
- A High-Efficiency Polarizing Interferometer for Far-Infrared Spectroscopy
- Variation of Bragg-Case Diffraction Curves of X-Rays from a Thin Silicon Crystal with Crystal Thickness
- TTP/HUS and vWF-CP
- A Dispersive Method of Measuring Extended X-Ray Absorption Fine Structure
- Curvature Denendence of Reflection Profiles of Silicon Curved Crystal Monochromators as Measured with Double-Crystal (n,-n) Setting : REFINED SPECTROSCOPY, X-RAY OPTICS AND INSTRUMENTATION
- X-Ray Crystal Collimators Using Successive Asymmetric Diffractions and Their Applications to Measurements of Diffraction Curves. : III. Type II Collimator
- GENETIC DIVERSITY OF Loropetalum chinense (R. Br.) Oliv. IN A NATURAL POPULATION
- Virus-Associated Hemophagocytic Syndrome Caused by Pandemic Swine-Origin Influenza A (H1N1) in a Patient After Unrelated Bone Marrow Transplantation
- Dexamethasone palmitate successfully attenuates hemophagocytic syndrome after allogeneic stem cell transplantation : macrophage-targeted steroid therapy
- Efficacy and safety of human adipose tissue-derived mesenchymal stem cells for supporting hematopoiesis