Establishment of New Highly Insulin-Sensitive Cell Lines and Screening of Compounds to Facilitate Glucose Consumption

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概要

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• To obtain compounds that promote glucose uptake in muscle cells, the novel cell lines A31-IS derived from Balb/c 3T3 A31 and C2C12-IS from mouse myoblast C2C12 were established. In both cell lines, glucose consumption was induced by insulin and suppressed by the addition of Akt-activating kinase inhibitor. The A31-IS cells highly express the insulin receptor β chains, Glut4, and uncoupling protein-3, as compared to the parent Balb/c 3T3 A31 cells, and C2C12-IS cells highly express the insulin receptor β chain as compared to its parent cell line. Using A31-IS cells, we screened our library compounds and obtained three compounds, DF-4394, DF-4451, and DG-5451. These compounds dose-dependently promoted glucose consumption in A31-IS cells and facilitated [3H]-2-deoxyglucose uptake in differentiated C2C12-IS cells. The compounds that we obtained from the library screening will be good candidates for improving insulin resistance in muscle cells.

• 社団法人 日本薬理学会の論文
• 2008-11-20

著者

• HAYATA Kenji

  R&D Division, Exploratory Research Laboratories II, Daiichi-Sankyo Co., Ltd.

• SAKANO Katsuichi

  R&D Division, Exploratory Research Laboratories I, Daiichi-Sankyo Co., Ltd.

• NISHINAKA Shigeyuki

  R&D Division, Exploratory Research Laboratories II, Daiichi-Sankyo Co., Ltd.

• Nishinaka Shigeyuki

  R&d Division Exploratory Research Laboratories Ii Daiichi-sankyo Co. Ltd.

関連論文

• A New binding Assay of von Willebrand Factor and Glycoprotein Ib Using Solid-Phase Biotinylated Platelets
• Establishment of New Highly Insulin-Sensitive Cell Lines and Screening of Compounds to Facilitate Glucose Consumption

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