N,N-Dimethyl-D-erythro-Sphingosine Inhibits Store-Operated Ca^<2+> Entry in U937 Monocytes
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概要
- 論文の詳細を見る
Calcium is a ubiquitous second messenger that controls a broad range of cellular functions, and store-operated calcium entry (SOCE) is the primary mechanism of regulated Ca2+ entry in non-excitable immunocytes. In this study, we found that N,N-dimethyl-<sc>D</sc>-erythro-sphingosine (DMS) inhibited SOCE. In U937 cells, treatment with DMS for 2 h inhibited thapsigargin-induced SOCE by about 70%. DMS inhibited SOCE in a concentration-dependent manner when it was added to the cells after SOCE reached a plateau. DMS-induced SOCE inhibition was also confirmed by the Mn2+-quenching method, which monitors only Ca2+ influx. Because sphingosine kinase inhibitors or protein kinase C (PKC) inhibitors could not mimic the SOCE inhibition, sphingosine kinase and PKC could be excluded as targets of DMS-induced inhibition of SOCE. Furthermore, disruption of lipid rafts with methyl-β-cyclodextrin and bacterial sphingomyelinase did not influence DMS-induced inhibition of SOCE. DMS-induced inhibition of SOCE in U937 human monocytes is a unique observation and could serve as a basis to study modulation of intracellular Ca2+ concentration by sphingolipids, although the precise mechanism should be elucidated in the future.
- 社団法人 日本薬理学会の論文
- 2008-07-20
著者
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Okajima Fumikazu
韓国
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Okajima F
Laboratory Of Signal Transduction Institute For Molecular And Cellular Regulation Gunma University
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BAE Yoe-Sik
Department of Biochemistry, Dong-A University College of Medidine
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Lee Yun-kyung
Laboratory Of Pharmacology College Of Pharmacy (bk21 Project) And Longevity Life Science And Technol
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OKAJIMA Fumikazu
Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University
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IM Dong-Soon
Laboratory of Pharmacology, College of Pharmacy and Research Institute for Drug Development, Pusan N
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TOMURA Hideaki
Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University
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Kim Hyo-lim
Laboratory Of Pharmacology College Of Pharmacy (bk21 Project) And Longevity Life Science And Technol
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Bae Yoe-sik
Department Of Biochemistry College Of Medicine Dong-a University
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Im D‐s
Laboratory Of Pharmacology College Of Pharmacy (bk21 Project) And Longevity Life Science And Technol
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Im Dong-soon
Laboratory Of Pharmacology College Of Pharmacy Pusan National University
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Tomura Hideaki
Laboratory Of Signal Transduction Institute For Molecular And Cellular Regulation Gunma University
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JO Ji-Yeong
Laboratory of Pharmacology, College of Pharmacy (BK21 Project) and Longevity Life Science and Techno
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Jo Ji-yeong
Laboratory Of Pharmacology College Of Pharmacy (bk21 Project) And Longevity Life Science And Technol
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Okajima Fumikazu
Laboratory Of Signal Transduction
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