Polymorphism of Genes Associated with Putative Fimbriae of Bifidobacterium longum Strains, with Specific Reference to Their Host Specific Colonization
スポンサーリンク
概要
- 論文の詳細を見る
The polymorphism of a length of DNA including several genes encoding putative mucosal glycoprotein binding fimbriae-associated proteins (the putative fimbriae region) of Bifidobacterium longum was investigated with specific reference to its host specific colonization. PCR-restriction fragment length polymorphism (RFLP) assays were performed on 44 clonally different strains that had been isolated from fecal samples collected from 12 Japanese subjects over a 15-month period. The assays revealed that the putative fimbriae region is highly heterogeneous, with only a limited number of strains sharing the same RFLP fragment pattern. However, fragment patterns observed for the adjacent up- and down-stream DNA regions not associated with the fimbriae shared the same fragment patterns in many strains, further highlighting the polymorphic nature of the putative fimbriae region. A dendrogram created of the fragment patterns of the putative fimbriae region showed 5 genotypes at the 70% similarity level, and 4 of the genotypes were further subdivided at the 80% similarity level into 9 sub-genotypes, in which at least half of the strains isolated from each host belonged to the same sub-genotype. The evidence suggests that fimbriae of B. longum are closely associated with the host-specific intestinal colonization. If this is the case, probiotic use of B. longum strains indigenous to the host may be more effective for the promotion and maintenance of that individuals health.
- 2008-04-01
著者
-
OSAWA Ro
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University
-
Nishitani Yosuke
Department Of Bioresource Science Graduate School Of Agricultural Science Kobe University
-
Osawa Ro
Department Of Bacteriology And Pathology Kanagawa Prefectural Public Health Laboratory
-
MAEGAWA Takahiro
Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University
-
Osawa Ro
Dep. Of Bioresource Sci. Graduate School Of Agricultural Sci. Kobe Univ.
-
Maegawa Takahiro
Department Of Bioresource Science Graduate School Of Agricultural Science Kobe University
関連論文
- Genetic analyses of the putative O and K antigen gene clusters of pandemic Vibrio parahaemolyticus
- Identification of Enterohemorrhagic Escherichia coli O157-Specific DNA Sequence Obtained from Amplified Fragment Length Polymorphism Analysis
- Identification of Vibrio parahaemolyticus Pandemic Group-Specific DNA Sequence by Genomic Subtraction
- PCR-Based Identification of Pandemic Group Vibrio parahaemolyticus with a Novel Group-Specific Primer Pair
- Genotypic Analyses of Vibrio parahaemolyticus and Development of a Pandemic Group-Specific Multiplex PCR Assay
- Spontaneous recombination between homologous prophage regions causes large-scale inversions within the Escherichia coli O157:H7 chromosome
- O side chain deficiency enhances sensitivity of Escherichia coli to Shiga toxin 2-converting bacteriophages
- Genotyping of pandemic Vibrio parahaemolyticus O3:K6 still open to question
- Effects of Lysogeny of Shiga Toxin 2-Encoding Bacteriophages on Pulsed-Field Gel Electrophoresis Fragment Pattern of Escherichia coli K-12
- Effects of Repeated Subculturing and Prolonged Storage at Room Temperature of Enterohemorrhagic Escherichia coli O157:H7 on Pulsed-Field Gel Electrophoresis Profiles
- Levels of Thermostable Direct Hemolysin Produced by Vibrio parahaemolyticus O3:K6 and Other Serovars Grown Anaerobically with the Presence of a Bile Acid
- Anti-Inflammatory Effect of Bifidobacterium longum on Macrophage-Like THP-1 Cells via Epithelial Cell Caco-2
- Different Utilization of Oligosaccharides and Distribution of Several Genes Associated with Oligosaccharide Metabolism in Bifidobacterium longum
- Polymorphism of Genes Associated with Putative Fimbriae of Bifidobacterium longum Strains, with Specific Reference to Their Host Specific Colonization
- Pulsed-Field Gel Electrophoresis and Lectin Blotting Analyses of Bifidobacterium longum Strains Isolated from Human Feces and Probiotic Products
- An Improved Enrichment Broth for Isolation of Escherichia coli O157, with Specific Reference to Starved Cells, from Radish Sprouts
- Growth of Starved Escherichia coli O157 Cells in Selective and Non-Selective Media
- A Note on Use of Brain Heart Infusion Broth Supplemented with Glucose and a Zwitterionic Buffer for Mass Culture of Stapylococcus aureus
- Deceptive Halo Formation by Tannase-Defective Bacteria on Tannin-Treated Plate Media
- Intra-specific composition and succession of Bifidobacterium longum in human feces
- Intra-Species Diversity between Seven Bifidobacterium adolescentis Strains Identified by Genome-Wide Tiling Array Analysis
- Genotypic Analyses of Escherichia coli Isolated from Chickens with Colibacillosis and Apparently Healthy Chickens in Japan
- Complexing of Green Tea Catechins with Food Constituents and Degradation of the Complexes by Lactobacillus plantarum
- Antimicrobial Susceptibility of Streptococcus gallolyticus Isolated from Humans and Animals