石綿の経気道生体影響とバイオマーカーの検索 : ヘムオキシゲナーゼ-1(HO-1)を中心に

概要

論文の詳細を見る
It is well known that asbestos induced pulmonary inflammation result in fibrosis. Oxidative stress is thought to be one of the pathogenesis of pulmonary fibrosis, and Heme Oxygenase-1 (HO-1) has been linked to process of protecting lung tissue against oxidative stress. Heme Oxygenase-1 (HO-1) inducible enzyme that catalyzes heme to generate bilirubin, ferritin, and carbon monoxide (CO) and these resolved products immediately protect lung tissue against oxidative stress. To determine whether or not HO-1 can act as a biomarker of lung injury induced by materials, we made serial measurements of HO-1 expression in rat lungs following the intratracheal instillation of several materials (crocidolite, chrysotile, amosite, crystalline silica) . Male Wistar rats were administered 1 mg or 2 mg of materials suspended in saline by a single intratrachally and were sacrificed at 3 days, 1 week, 1 month, 3 months and 6 months of recovery time. The expression of HO-1 in the rat lung was observed by western blot or ELISA analysis. In case of crocidolite, amosite, chrysotile and crystalline silica which cause sever injury, protein levels of HO-1 in the rat lung constantly increased during the recovery time. The pattern of HO-1 expression is different from materials with severe lung injury and mild or moderate lung injury. It suggests that HO-1 can act as a biomarker of lung injury exposed to particles and fibrous materials.
日本エアロゾル学会の論文
2008-03-20