Micro-Plate Based Monolithic Ion-Exchange Chromatography for High Throughput Protein Purification Process Design
スポンサーリンク
概要
- 論文の詳細を見る
As a method for surveying efficient separation conditions for the mobile phase, a 96-well micro-plate based monolithic ion-exchange chromatography system was developed. Discontinuous flow was accomplished by a vacuum manifold. Linear salt gradient elution experiments were carried out with this system and the elution curves were compared with those for monolithic disk column IEC. The normalized gradient slope–the peak salt concentration data for the micro-plate system agreed with those for the monolithic disk column IEC. This system is useful as the liquid volume needed for the experiment is highly reduced, and large amounts of data are obtained quickly.
- 社団法人 化学工学会の論文
- 2008-03-01
著者
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Yamamoto Shuichi
Yamaguchi University
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Fujii Sachie
Yamaguchi University
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Yoshimoto Noriko
Yamaguchi University
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Abe Mitsuyo
Yamaguchi University
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YOSHIMOTO Noriko
Laboratory of Bioprocess Engineering, School of Engineering and Graduate School of Medicine, Yamaguc
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NISHIJIMA Yuko
Laboratory of Bioprocess Engineering, School of Engineering and Graduate School of Medicine, Yamaguc
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AKBARZADEHLALEH Parvin
Laboratory of Bioprocess Engineering, School of Engineering and Graduate School of Medicine, Yamaguc
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FUJII Sachie
Laboratory of Bioprocess Engineering, School of Engineering and Graduate School of Medicine, Yamaguc
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ABE Mitsuyo
Laboratory of Bioprocess Engineering, School of Engineering and Graduate School of Medicine, Yamaguc
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YAMAMOTO Shuichi
Laboratory of Bioprocess Engineering, School of Engineering and Graduate School of Medicine, Yamaguc
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Akbarzadehlaleh Parvin
Laboratory Of Bioprocess Engineering School Of Engineering And Graduate School Of Medicine Yamaguchi
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Nishijima Yuko
Laboratory Of Bioprocess Engineering School Of Engineering And Graduate School Of Medicine Yamaguchi
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