Changes in the Expression of Toll-Like Receptor mRNAs During Follicular Growth and in Response to Lipopolysaccharide in the Ovarian Follicles of Laying Hens
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概要
- 論文の詳細を見る
The aim of this study was to determine the changes in the mRNA expression of Toll-like receptors (TLRs) in hen ovarian follicles during follicular growth and in response to lipopolysaccharide (LPS). White follicles and the fifth largest to largest follicles (WF and F5-F1, respectively) were collected from laying hens. To examine the effects of LPS, the laying hens were treated intravenously with LPS (1 mg/kg BW) 0, 3, 6, 12 and 24 h before examination. Expressions of TLRs and IL-1 β in the theca and granulosa layers were examined by semi-quantitative RT-PCR. Immunocytochemistry was performed to identify immunoreactive TLR-4. The theca layer expressed TLR-2, TLR-4, TLR-5 and TLR-7, whereas the granulosa layer expressed only TLR-4 and TLR-5. The expression of TLR-4 and TLR-5 in the theca layer increased significantly during follicular growth. In the granulosa layer, the expression of TLR-5 increased, but that of TLR-4 was unchanged. Expression of TLR-4 increased significantly during the period of 6 to 12 h of LPS treatment in the theca layer and during the period of 12 to 24 h in the granulosa layer of F3. Immunoreaction products for TLR-4 were observed in theca interna and granulosa layers of WF and F5-F1, with the greater amount observed in the theca interna. LPS treatment significantly increased expression of IL-1β in the theca layer after 3 h and in the granulosa layer during the period of 12 to 24 h. These results suggest that TLRs are expressed in ovarian follicles and that TLR-4 and TLR-5 expression increases with the growth of follicles. Enhanced expression of TLR-4 and IL-1β by LPS in the theca and granulosa layers suggests possible roles of TLR in recognition of microorganisms.
- 日本繁殖生物学会の論文
- 2007-12-01
著者
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Isobe Naoki
Graduate School Of Biosphere Sci. Hiroshima Univ. Jpn
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Subedi Kalpana
Graduate School Of Biosphere Sci. Hiroshima Univ. Jpn
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YOSHIMURA Yukinori
Graduate School of Biosphere Science, Hiroshima University
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Isobe Naoki
Graduate School Of Biosphere Science Hiroshima University
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Isobe Naoki
Graduate School For International Development And Cooperation Hiroshima University
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NISHIBORI Masahide
Graduate School of Biosphere Science, Hiroshima University
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Nishibori Masahide
Graduate School Of Biosphere Hiroshima University
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Yoshimura Yukinori
Graduate School Of Biosphere Sci. Hiroshima Univ. Jpn
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