Expression and Characterization of the Genes Encoding Azoreductases from Bacillus subtilis and Geobacillus stearothermophilus
スポンサーリンク
概要
- 論文の詳細を見る
Azoreductases have been characterized as enzymes that can decolorize azo dyes by reducing azo groups. In this study, genes encoding proteins having homology with theazoreductase gene of Bacillus sp. OY1-2 were obtained from Bacillus subtilis ATCC6633, B. subtilis ISW1214, and Geobacillus stearotherophilus IFO13737 by polymerase chain reaction. All three genes encoded proteins with 174 amino acids. The deduced amino acid sequences of azoreductase homologs from B. subtilis ISW1214, B. subtilis ATCC6633, and G. stearotherophilus IFO13737 showed similarity of 53.3, 53.9, and 53.3% respectively to that of Bacillus sp. OY1-2.All three genes were expressed in Escherichia coli, and were characterized as having the decolorizing activity of azo dyes in a β-NADPH dependent manner. The transformation of several azo dyes into colorless compounds by recombinant enzymes was demonstrated to have distinct substrate specificity from that of azoreductase from Bacillus sp. OY1-2.
- 社団法人 日本農芸化学会の論文
- 2006-07-23
著者
-
Tanaka Yoshinori
Department of Medicine, Yamaguchi Prefectural Medical Center
-
Suzuki Yasuhiko
Department of Microbiology and Immunochemistry, Asahikawa Medical College
-
Sugiura Wataru
Department Of Environmental Health Osaka Prefectural Institute Of Public Health
-
Yoda Tomoko
Infectious Desieses Osaka Prefectural Institute Of Public Health
-
MATSUBA Takashi
Department of Microbiology and Pathology, Tottori University Faculty of Medicine
-
Tanaka Yoshinori
Department Of Bacteriology Faculty Of Medicine Tottori University
-
Tanaka Yoshinori
Department Of Microbiology And Pathology Tottori University Faculty Of Medicine
-
Yoda Tomoko
Infectious Desieses Osaka Prefectural Inst. Of Public Health
-
Matsuba Takashi
Department Of Microbiology And Pathology Tottori University Faculty Of Medicine
-
Matsuba Takashi
Department Of Microbiology And Pathology Tottori University
-
Suzuki Yasuhiko
Department Of Global Epidemiology Hokkaido University Research Center For Zoonosis Control
-
SUZUKI Yasuhiko
Department of Chemistry, Shizuoka University
関連論文
- T-γ δ Large Granular Lymphocyte Leukemia Preceded by Pure Red Cell Aplasia and Complicated with Hemophagocytic Syndrome Caused by Epstein-Barr Virus Infection
- CL-P1, a Newly Cloned Endothelial Cell Specific Scavenger Receptor
- Haplotype analysis of the human collectin placenta 1 (hCL-P1) gene
- Morphological and cytological aspects of oidium formation in a basidiomycete, Pholiota nameko
- Is the short-term outcome of transurethral resection of the prostate affected by preoperative degree of bladder outlet obstruction, status of detrusor contractility or detrusor overactivity?
- Intramolecular "Through-Bond" and "Through-Space" Electron Transfer Pathways in Covalently Linked Porphyrin-Quinone Molecules^
- Synthetic Model Approach to "Through-Bond" and "Through-Space" Electron Transfer Pathways
- Porphyrin-Quinone Compounds with a Spacer Diacetylene Unit
- Esophageal tuberculosis presenting with an appearance similar to that of carcinoma of the esophagus
- Green Photoluminescence from GaInN Photonic Crystals
- Efficiency of Rebamipide in the Prevention of Remnant Gastritis after Distal Gastrectomy
- Identification and Characterization of a Novel Human Collectin CL-K1
- Contractille properties of urethral smooth muscles of young and aged female dogs: Morphological and pharmacological aspects
- Self-Expandable Metallic Stent for Unresectable Malignant Strictures in the Esophagus and Cardia
- Ambulatory intraesophageal bilirubin monitoring in Japanese patients with gastroesophageal reflux
- Fucoxanthin-Pyropheophorbide and Zeaxanthin-Pyropheophorbide Dyads as New Models for Study on Carotenoid-Chlorophyll Excited State Interactions
- Sequential Electron Transfer Leading to Long-Lived Charge Separated State in a Porphyrin-Oxochlorin-Pyromellitdimide Triad
- Warming and Sterilizing Towels by Microwave Irradiation
- イシマキガイとアマオブネの血液細胞への腸炎ビブリオの付着〔英文〕
- Different Sensitivity to Streptolysin-O of Cells in Macrophage Lineage
- Biological Activities of YTIT/YTIY Analogous Sequences Present in Fibronectin-Like Type III Repeats
- Malignant mesothelioma of the tunica vaginalis testis : A case with a predominant sarcomatous component
- Hematospermia:An investigation of the bleeding site and underlying lesions The cause of hematospermia
- Hemangioma of the Prostatic Urethra:Hematospermia and Massive Postejaculation Hematuria with Clot Retention
- Endoscopic Laser Ureterolithotripsy for Outpatients
- On-the-Fly Wavelength Conversion of Photons by Dynamic Control of Photonic Waveguides
- Oxidative Depolymerization of Chitosan by Hydroxyl Radical
- Urodynamic effects of solifenacin in untreated female patients with symptomatic overactive bladder
- X-RAY STUDY OF SO-CALLED SPONTANEOUS HEALING IN CONGENITAL DISLOCATION OF HIP-JOINT
- Expression and Characterization of the Genes Encoding Azoreductases from Bacillus subtilis and Geobacillus stearothermophilus
- Characterization of Truncated Human Mannan-Binding Protein (MBP) Expressed in Escherichia coli
- The combination of aztreonam and cefozopran against Stenotrophomonas maltophilia
- EXPERIENCES ON ULTRASONIC IRRADIATION OF THE CAROTID GLAND HYPERTENSION THERAPY
- Regulation of Promoter and Intron Enhancer Activity in Immunoglobulin Heavy-Chain Genes during B-Cell Differentiation
- Induction of Apoptosis in Macrophage Cell Line, J774, by the Cell-Free Supernatant from Pseudomonas aeruginosa
- Enhancement of Legionella pneumophila Culture Isolation from Microenvironments by Macrophage Infectivity Potentiator (mip) Gene-Specific Nested Polymerase Chain Reaction
- Activation of the unfolded protein response in primary acute myeloid leukemia cells
- Survival of Bacteria at a Subfreezing Temperature (－1°C)
- Role of Pseudomonas aeruginosa Culture Filtrates in the Association, Invasion, and Cytotoxicity Against Cloned Cells from Murine Corneal Epithelium and KB Cells
- Establishment of an indicator cell system for hepatitis C virus
- Mass Spectrometric Study of C2-Hydrocarbons: : Oxidation in Shock Waves
- Distinct Clinical Features in Nontuberculous Mycobacterial Disease with or without Latent Tuberculosis Infection
- Antibiotic Susceptibility of Staphylococcus spp. Collected from the Entrance Hall of the New Dispensary at Tottori University Hospital
- Epidemiological Surveys of Methicillin-Resistant Staphylococcus aureus in Tottori Prefecture, Japan
- Recurrence of Hepatitis-Associated Aplastic Anemia after a 10-year Interval
- Anti-Influenza A Virus Activities of Mannan-Binding Lectins and Bovine Conglutinin
- Scanning Electron Microscopic and Cytochemical Analyses of Alveolar and Peritoneal Macrophages of Mice, Dogs and Cats.
- Rapid Preparation of Plasmid by Gel Permeation Chromatography on Toyopearl HW75S
- KARYOTYPE AND G6PD ISOZYME PATTERN OF A CELL LINE DERIVED FROM HUMAN ENDOMETRIUM
- Changes in perioperative management of radical prostatectomy using clinical pathways according to a standardized care plan : A multi-institutional study
- Comparison of human blood concentrations of collectin kidney 1 and mannan-binding lectin