シリンジ型オフラインプレカラムとHPLCを用いた生体試料からのコハク酸プレドニゾロンとプレドニゾロンの迅速分析

概要

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The determination of prednisolone-hemisuccinate (PSL-HS) and prednisolone (PSL) were performed using high-performance liquid chromLutography (HPLC). PSL-HS and PSL were simultaneously extracted and injected onto a silica gel column with a syringe-type minicolumn packed with diatomaceous earth granules. The extrilction-injection solvent used was dichloromethane containing 1% ethanol and the mobile phase solvent tbr HPLC was water-acetic acid-methLlnol-dichloromethane-n-hexane (0.2 : 0.2 : 4 : 95.6,v/v). PSL-HS and PSL were detected using an ultraviolet detector set at 250 nm. Linear relationships between the amount of sample and peak height were confirmed from 0.1 to 100μg/ ml of homogenate of rat rectum and humiln serum. When an aliquot of 5μl of sample was introduced to the syringe-type minicolumn, the detection limits of PSL-HS and PSL were as low as 100 ng/ml with each. The method is simple and accurate and is thus applicable to pharmacology and pharmacokinetic studies.
日本医療薬学会の論文
1997-10-10