A Novel Genetic System for Analysis of Co-activators for the N-Terminal Transactivation Function Domain of the Human Androgen Receptor

スポンサーリンク

概要

• 論文の詳細を見る

• Androgen receptor (hAR) regulates transcription of target genes in a ligand-dependent manner and recruits a number of co-activators for the ligand-induced transactivation via the N-terminal, activation function-1 (AF-1), and C-terminal, AF-2, transactivation domains. But the co-regulator functions on each of AR domains have not yet been fully understood. We have established a Drosophila transgenic system in which hAR and its deletion mutants are ectopically expressed in fly tissues together with an AR response element (ARE)-GFP reporter gene, and have confirmed that hAR was functional in ARE transactivation without affecting the expression of endogenous genes. We found that transcriptional activity of the hAR AF-1 domain was markedly reduced in Drosophila deficiency mutants of homologs for known mammalian co-activators of the AR ligand-dependent AF-2 domain. This suggests that hAR AF-1 recruits co-activators previously known only to interact with the AF-2 domain. Therefore, Drosophila with the hAR AF-1 transgene provides a relevant genetic system in which to uncover novel functions of vertebrate steroid hormone receptors and to screen for novel AF-1 co-regulators.

• 社団法人 日本農芸化学会の論文
• 2004-06-23

著者

• Kato Shigeaki

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• TAKEYAMA Ken-ichi

  Institute of Molecular and Cellular Biosciences, The University of Tokyo

• Takeyama Ken-ichi

  Inst. Of Molecular And Cellular Biosciences Univ. Of Tokyo

• Takeyama Ken-ichi

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Kato Shigeaki

  Institute Of Molecular And Cellular Bioscience University Of Tokyo

• Kato Shigeaki

  Exploratory Res. For Advanced Technol. (erato) Japan Sci. And Technol. Agency (jst)

• Zhao Yue

  Inst. Of Molecular And Cellular Biosciences Univ. Of Tokyo

• Tabata Tetsuya

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Yamamoto A

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• ITO Saya

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• SUZUKI Eriko

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• SAWATSUBASHI Shun

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• SHIRODE Yuko

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• YAMAMOTO Ayako

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• MAKI Akio

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• YAMAGATA Kaoru

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• ZHAO Yue

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• KOUZMENKO Alexandre

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• Suzuki Eriko

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Yamagata Kaoru

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Shirode Yuko

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Ito Saya

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Zhao Yue

  Institute Of Mobile Communications In Southwest Jiaotong University

• Sawatsubashi Shun

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Kouzmenko Alexandre

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Tabata Tetsuya

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• Maki Akio

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Kato Shigeaki

  Institute for Chemical Research, Kyoto University

関連論文

• PJ-070 Exaggerated Atherosclerosis in Heparin Cofactor II-Deficient ApoE Knockout Mice(Atherosclerosis, basic-8, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• OJ-216 Acceleration of Angiotensin II-Induced Coronary Artery Remodeling in Androgen Receptor Knockout Male Mice(Atherosclerosis, basic-2 (H) OJ36,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Soci
• OE-127 Androgen-Androgen Receptor System Counteracts Doxorubicin-Induced Cardiotoxicity through Attenuating Oxidative Stress-Induced Mitochondrial Damage in Murine Male Heart(Heart failure, basic-1 (M) OE22,Oral Presentation (English),The 70th Anniversary
• Androgen Receptor Gene Knockout Male Mice Exhibit Exacerbated Cardiotoxicity and Increased Mortality by Doxorubicin Administration(Cardiac Function, Basic/Clinical 1 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Arterial Cuff Injury Causes Enhanced Neointimal Formation and Increased Adventitial Area in Heterozygous Heparin Cofactor II Deficient Mice(Atherosclerosis, Basic 1 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• PJ-359 Impaired Cardiac Hypertrophic Response and Exacerbated Cardiac Fibrosis in Angiotensin Il-Loaded Male Mice Lacking Androgen Receptor(Molecular Biology, Myocardium 1 (M) : PJ60)(Poster Session (Japanese))
• OJ-373 Vitamin D/Vitamin D Receptor System Regulates Antithrombogenicity in vivo(Thromboembolism/Thrombolysis 3 (IHD) : OJ45)(Oral Presentation (Japanese))
• Heparin Cofactor II Is Inversely Related to the Severity of Carotid Atherosclerosis
• Elevated blood pressure with a cardiovascular remodeling in mice lacking vitamin D receptor
• The Vitamin D3 1alpha-Hydroxylase Gene and Its Regulation by Active Vitamin D3
• Nuclear Receptor Mediated Gene Regulation through Chromatin Remodeling and Histone Modifications
• ApoB C7623T polymorphism predicts risk for steroid-induced osteonecrosis of the femoral head after renal transplantation
• Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
• Transcriptional Controls by Nuclear Fat-Soluble Vitamin Receptors through Chromatin Reorganization
• Dietary quercetin inhibits bone loss without effect on the uterus in ovariectomized mice
• FGF10 AND GATA4 ARE INVOLVED IN THE DEVELOPMENT OF THE GLANDULAR STOMACH OF FETAL MOUSE(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
• Steroid Hormones Differentially Induce Transcription of the Chicken Ovalbumin Gene, But Stabilize the mRNA with the Same Half-Life
• The Molecular Basis of Vitamin D-Dependent Rickets Type I
• Missense Mutations in 25(OH) Vitamin D_3 1α-Hydroxylase Gene Causes Vitamin D Dependent Type I Rickets
• A Role of Androgen Receptor Protein in Cell Growth of an Androgen-Independent Prostate Cancer Cell Line
• A Novel Genetic System for Analysis of Co-activators for the N-Terminal Transactivation Function Domain of the Human Androgen Receptor
• Nuclear Receptors as Targets for Drug Development : Crosstalk Between Peroxisome Proliferator-Activated Receptor γ and Cytokines in Bone Marrow-Derived Mesenchymal Stem Cells
• The function of nuclear receptors in bone tissues
• Androgen receptor functions in male and female reproduction
• Study of androgen action in bone by analysis of androgen-receptor deficient mice
• The Vitamin D3 1alpha-Hydroxylase Gene and Its Regulation by Active Vitamin D3
• RNA-Binding Protein Hoip Accelerates PolyQ-Induced Neurodegeneration in Drosophila
• Study of Androgen Receptor Functions by Genetic Models
• An Energy Saving Scheduling Scheme for OFDMA Two-Hop Relay Systems
• Robustness of the Dpp morphogen activity gradient depends on negative feedback regulation by the inhibitory Smad, Dad
• Transcriptional Controls by Nuclear Fat-Soluble Vitamin Receptors through Chromatin Reorganization
• Fat/Hippo pathway regulates the progress of neural differentiation signaling in the Drosophila optic lobe
• Lesson from Androgen Receptor Knock-out (ARKO) Mice
• Androgen Receptor Structure and Function from Knock-out Mouse
• The Function of Vitamin D Receptor in Vitamin D Action
• The New Dimension of Endocrinology : Nuclear/Intracellular Endocrinology
• Williams syndrome is an epigenome-regulator disease
• Retraction: Nuclear Receptors as Targets for Drug Development: Crosstalk Between Peroxisome Proliferator-Activated Receptor γ and Cytokines in Bone Marrow-Derived Mesenchymal Stem Cells
• Employment of the Human Estrogen Receptor .BETA. Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters.

もっと見る 閉じる

スポンサーリンク